BACILLUS SUBTILIS PROTEIN EXPRESSION SERVICE

You are looking for an endotoxin-free expression system? Opt for a safe E.coli alternative with our Bacillus subtilis expression system. Benefit now from a tailored protein expression
protocol thanks to a pilot study ahead of large-scale manufacturing.

Small-Scale Development in Bacillus Subtilis Protein Expression Service

An efficient protocol established for one specific protein, will not guarantee the same efficiency for all other proteins. For this reason, our service starts with a pilot study. The aim is to test several parameters on a small-scale to determine the optimal condition to produce the targeted protein. The best expression parameters are selected for large-scale production to meet your expectations with your desired protein quantity.

The standard pilot study features 16 conditions to express a recombinant protein with 3 variables:

• 4 B.subtilis strains.
• 2 Temperatures : 30°C and 37°C.
• 2 Plasmid fractions : Linear and native.

After selecting the optimum conditions, further purification tests are performed to figure out the highest possible recombinant protein yields. The protocol is then up-scaled to 1L protein production using the selected conditions.

A difficult-to-express protein? Optimize your chances to express it thanks to our high throughput protein expression screening service.

Conclusion of the HTP expression screening in Bacillus subtilis:

Thanks to high throughput expression screening, the protein can now be produced in native and soluble conditions with an expression yield of up to ~30mg/L in standard conditions.

Why choose Bacillus Subtilis for your Protein Expression?

Belonging to Bacillus species, Bacillus subtilis is a well-studied gram-positive bacterium. It has become a popular protein factory host for industrial manufacturing. It presents significant attributes in comparison with gram-negative bacteria such as E.coli.

•  B.subtilis Protein Expression as an Alternative to E.coli:

In terms of large-scale production, Bacillus subtilis constitutes an easy and inexpensive organism to culture. As it is genetically well characterized, it simplifies genetic manipulation. Plus, it is known for its rapid proliferation rates.

Furthermore, Bacillus subtilis is recognized as safe. It acquired the GRAS status (Generally Recognized as Safe) by the US Food and Drug Administration.

• Simple Downstream Processing:

Thanks to its efficient secretory pathways, B.subtilis can export expressed proteins directly into the culture media. Newly synthesized proteins can acquire proper folding and prevent protein accumulation and inclusion body formation. Therefore, the cell naturally avoids intracellular toxicity by isolating proteins in the extracellular environment. Unlike gram-negative bacteria, B.subtilis are deprived of endotoxins. This simplifies the downstream process in cases where the final product must be endotoxin-free.

• Applications of Bacillus Subtilis as an Expression System:

Bacillus subtilis occupies an important place in several areas, including the medical, agro-food, and ecological fields. It constitutes a cell factory for therapeutical heterologous protein proteins, amino acids, bioactive molecules, and vaccines.

It is also the preferred prokaryotic organism in the biopharmaceutical and agro-food fields. 60% of enzyme production in the industry is established using B.subtilis such as proteases, amylases, lipases, and galactosidase. Furthermore, human interferon-β/ γ and interferon-α 2b and other membrane proteins are better produced in B.subtilis than in E.coli. Even though protein yields are not as high as E.coli, better protein folding is accomplished with B.subtilis. thanks to their slower translation rates.

Please feel free to contact us for further information!