Recombinant Human PPP1CA, N-His

Description of Recombinant Human PPP1CA, N-His

Introduction

Recombinant proteins have become essential tools in various fields of research and biotechnology. They are produced by inserting a gene encoding the desired protein into a host organism, allowing for large-scale production and purification. One such recombinant protein is Recombinant Human PPP1CA, which has been extensively studied for its structure, activity, and potential applications.

Structure of Recombinant Human PPP1CA

Recombinant Human PPP1CA, also known as protein phosphatase 1 catalytic subunit alpha, is a 37 kDa protein that belongs to the PPP family of serine/threonine phosphatases. It is encoded by the PPP1CA gene located on chromosome 11 in humans. The protein consists of 330 amino acids and contains a catalytic domain, a regulatory domain, and a C-terminal domain.

The catalytic domain of Recombinant Human PPP1CA is responsible for its phosphatase activity, which involves the dephosphorylation of serine and threonine residues on target proteins. The regulatory domain helps to regulate the activity of the catalytic domain, while the C-terminal domain is involved in protein-protein interactions.

Activity of Recombinant Human PPP1CA

As a serine/threonine phosphatase, Recombinant Human PPP1CA plays a crucial role in various cellular processes, including cell cycle regulation, signal transduction, and metabolism. It is involved in the dephosphorylation of key regulatory proteins, such as glycogen synthase, c-Myc, and tau, which are essential for proper cell function.

Studies have shown that Recombinant Human PPP1CA is highly specific for its substrates and exhibits optimal activity at a neutral pH. Its activity can be modulated by various factors, including metal ions, regulatory subunits, and post-translational modifications. Dysregulation of PPP1CA activity has been linked to various diseases, such as cancer, neurodegenerative disorders, and metabolic disorders.

Applications of Recombinant Human PPP1CA

Recombinant Human PPP1CA has been widely used as a research tool for studying the role of protein phosphatases in cellular processes. Its high specificity and activity make it a valuable tool for investigating the dephosphorylation of specific target proteins. It has also been used in drug discovery and development, as PPP1CA is a potential therapeutic target for various diseases.

One of the most promising applications of Recombinant Human PPP1CA is in the production of vaccines. It has been used as an antigen in vaccine development for diseases such as malaria, hepatitis B, and HIV. The protein’s ability to induce an immune response makes it a potential candidate for the development of novel vaccines.

Furthermore, Recombinant Human PPP1CA has also been used in the production of biosensors for the detection of various biomolecules. Its high specificity and activity make it a suitable candidate for the development of sensitive and accurate biosensors for diagnostic purposes.

Conclusion

In conclusion, Recombinant Human PPP1CA is a valuable recombinant protein that has been extensively studied for its structure, activity, and potential applications. Its role in cellular processes, potential therapeutic applications, and use as an antigen and biosensor make it a versatile tool for research and biotechnology. Further studies on this protein will continue to expand our understanding of its structure and function, leading to the development of new and innovative applications.