Recombinant Human ABCF2, N-His

Description of Recombinant Human ABCF2, N-His

Introduction to Recombinant Human ABCF2

Recombinant Human ABCF2 is a protein that is artificially produced in a laboratory using genetic engineering techniques. It belongs to the ATP-binding cassette (ABC) superfamily of transporters and is also known as ATP-binding cassette sub-family F member 2.

Structure of Recombinant Human ABCF2

The structure of Recombinant Human ABCF2 is composed of two transmembrane domains and two nucleotide-binding domains. The transmembrane domains are responsible for the transport of molecules across the cell membrane, while the nucleotide-binding domains are involved in the binding and hydrolysis of ATP, which provides the energy for transport.

The transmembrane domains of Recombinant Human ABCF2 are made up of six alpha-helices, which form a barrel-like structure. These helices are hydrophobic in nature and help to anchor the protein in the cell membrane. The nucleotide-binding domains, on the other hand, are composed of two subdomains, each containing a Walker A and Walker B motif. These motifs are involved in the binding and hydrolysis of ATP.

Activity of Recombinant Human ABCF2

Recombinant Human ABCF2 is a transporter protein that plays a crucial role in the transport of molecules across the cell membrane. It is primarily involved in the transport of lipids, such as cholesterol and phospholipids, as well as drugs and other toxins out of the cell.

The activity of Recombinant Human ABCF2 is dependent on the binding and hydrolysis of ATP. When ATP binds to the nucleotide-binding domains, a conformational change occurs in the protein, allowing it to bind to the molecule to be transported. This is followed by the hydrolysis of ATP, which provides the energy for the transport of the molecule across the cell membrane.

Recombinant Human ABCF2 is also involved in the transport of molecules within the cell, such as from the endoplasmic reticulum to the Golgi apparatus. This is important for the proper functioning of the cell and maintaining homeostasis.

Application of Recombinant Human ABCF2

Recombinant Human ABCF2 has a wide range of applications in both research and medicine. Its role in the transport of lipids and drugs makes it a potential target for the development of new therapies for diseases such as cancer, where abnormal lipid metabolism is often observed.

In research, Recombinant Human ABCF2 is used to study the mechanisms of transport across the cell membrane. It is also used to investigate the role of ABC transporters in drug resistance, as overexpression of ABC transporters is often associated with drug resistance in cancer cells.

Recombinant Human ABCF2 is also used in the production of vaccines. It can be used as an antigen to stimulate the immune system and generate an immune response, which can then be used to protect against certain diseases.

Conclusion

In summary, Recombinant Human ABCF2 is a transporter protein that plays a crucial role in the transport of molecules across the cell membrane. Its structure, activity, and applications make it a valuable tool in both research and medicine. Further studies on this protein may lead to the development of new therapies for various diseases and improve our understanding of the mechanisms of transport in the cell.