Recombinant Human ANGPTL3, N-His

Description of Recombinant Human ANGPTL3, N-His

The Structure of Recombinant Human ANGPTL3

Recombinant Human ANGPTL3 is a protein that is produced through genetic engineering techniques, specifically through the process of recombinant DNA technology. This involves inserting the gene for ANGPTL3 into a host organism, such as bacteria or yeast, which then produces the protein in large quantities. The resulting recombinant protein is identical to the natural human ANGPTL3 protein and has the same structure and function.

The ANGPTL3 protein is composed of 460 amino acids and has a molecular weight of approximately 53 kDa. It is made up of two distinct domains, an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain. These domains are connected by a linker region and are responsible for the protein’s function.

The Activity of Recombinant Human ANGPTL3

The main function of ANGPTL3 is to regulate lipid metabolism in the body. It does this by inhibiting the activity of enzymes involved in the breakdown of triglycerides and cholesterol, leading to reduced levels of these lipids in the blood. This activity is important for maintaining healthy levels of lipids and preventing diseases such as atherosclerosis and hyperlipidemia.

Recombinant Human ANGPTL3 has been shown to have the same inhibitory activity as the natural protein. Studies have demonstrated that it can effectively inhibit the activity of lipases, such as lipoprotein lipase and endothelial lipase, which are responsible for breaking down triglycerides and cholesterol, respectively. This results in a decrease in the levels of these lipids in the blood.

In addition to its lipid-regulating activity, ANGPTL3 has also been found to have anti-inflammatory and anti-angiogenic properties. It can inhibit the production of pro-inflammatory cytokines and prevent the formation of new blood vessels, which is important for controlling inflammation and tumor growth.

Applications of Recombinant Human ANGPTL3

The potential applications of Recombinant Human ANGPTL3 are vast and varied. One of the main uses of this protein is in the development of therapeutics for lipid-related disorders. By inhibiting the activity of lipases, ANGPTL3 can help to lower blood lipid levels and reduce the risk of cardiovascular diseases. It has also been studied as a potential treatment for other metabolic disorders, such as diabetes and obesity.

In addition to its therapeutic applications, Recombinant Human ANGPTL3 is also used in research and diagnostic applications. It can be used as an antigen in immunoassays to detect the presence of ANGPTL3 in biological samples. This is useful for studying the role of ANGPTL3 in various diseases and for monitoring its levels in patients.

Furthermore, Recombinant Human ANGPTL3 has potential applications in the field of biotechnology. It can be used in the production of transgenic animals with altered lipid metabolism, which can be used for studying the effects of ANGPTL3 on lipid regulation. It can also be used in the development of novel drug delivery systems, as well as in the production of recombinant vaccines.

In conclusion, Recombinant Human ANGPTL3 is a promising protein with a wide range of potential applications. Its structure and activity are similar to the natural protein, making it an effective tool for regulating lipid metabolism and potentially treating various diseases. With ongoing research and development, the full potential of this protein is yet to be realized.