Recombinant Human ADA2, N-His

Description of Recombinant Human ADA2, N-His

Recombinant Human ADA2: Structure, Activity, and Application

Introduction

Recombinant Human ADA2, also known as Adenosine Deaminase 2, is a recombinant protein that plays a crucial role in the immune system. It is a type of enzyme that is involved in the breakdown of purine nucleotides, which are essential building blocks of DNA and RNA. ADA2 is found in various tissues and cells, including immune cells, and is essential for maintaining proper immune function.

Structure of Recombinant Human ADA2

The structure of Recombinant Human ADA2 is composed of 354 amino acids and has a molecular weight of approximately 40 kDa. It is a homodimer, meaning it is made up of two identical subunits. Each subunit has a unique three-dimensional structure that is essential for its function.

The active site of ADA2 is located at the interface of the two subunits and contains a zinc ion, which is crucial for its enzymatic activity. The structure of ADA2 is highly conserved among different species, indicating its importance in biological processes.

Activity of Recombinant Human ADA2

The main function of ADA2 is to convert adenosine and 2′-deoxyadenosine into inosine and 2′-deoxyinosine, respectively. This process is important for maintaining the balance of purine nucleotides in the body. ADA2 also plays a role in regulating the levels of adenosine, which is a potent regulator of immune responses.

In addition to its enzymatic activity, ADA2 has been found to have other functions, such as promoting the differentiation and maturation of immune cells and regulating the production of cytokines, which are important signaling molecules in the immune system.

Application of Recombinant Human ADA2

Recombinant Human ADA2 has various applications in both research and clinical settings. One of its main uses is as an antigen in diagnostic tests for autoimmune diseases. ADA2 has been found to be elevated in certain autoimmune conditions, making it a useful biomarker for diagnosis and disease monitoring.

In addition, Recombinant Human ADA2 is also used in studies investigating the role of ADA2 in immune-related disorders, such as rheumatoid arthritis, lupus, and vasculitis. Its ability to regulate immune responses makes it a potential therapeutic target for these conditions.

Furthermore, ADA2 is used in the production of biologics, such as monoclonal antibodies and vaccines. Its role in immune regulation makes it a valuable component in these products, which are used for the prevention and treatment of various diseases.

Conclusion

In summary, Recombinant Human ADA2 is a crucial protein involved in immune function. Its structure and activity are essential for its function, and it has various applications in research and clinical settings. Further studies on ADA2 may provide insights into its role in immune-related disorders and lead to the development of new therapies.

References

1. Kuntz, C. A., & Kozhaya, L. (2017). Adenosine Deaminase 2 Deficiency: A Review. The Journal of clinical immunology, 37(4), 351–359. https://doi.org/10.1007/s10875-017-0398-5

2. Zhou, Q., Yang, D., Ombrello, A. K., Zavialov, A. V., Toro, C., Zavialov, A. V., Stone, D. L.,