Recombinant Human CDC25C Protein, N-His

Description of Recombinant Human CDC25C Protein, N-His

Introduction

The recombinant human CDC25C protein is a highly purified and biologically active protein that is produced using the latest recombinant DNA technology. It plays a crucial role in cell cycle regulation and is an essential component of the mitotic checkpoint. In this article, we will delve into the structure, activity, and applications of this important protein.

Structure of Recombinant Human CDC25C Protein

The recombinant human CDC25C protein is a 45 kDa protein consisting of 380 amino acids. It belongs to the CDC25 family of phosphatases, which are key regulators of the cell cycle. The protein has a conserved catalytic domain, which is responsible for its phosphatase activity. It also contains a C-terminal regulatory domain that mediates its interaction with other proteins involved in cell cycle regulation.

The crystal structure of the recombinant human CDC25C protein has been determined, revealing a compact globular structure with a central beta-sheet surrounded by alpha-helices. This structure is highly conserved across species, highlighting the importance of this protein in cell cycle regulation.

Activity of Recombinant Human CDC25C Protein

The main function of the recombinant human CDC25C protein is to activate the cyclin-dependent kinases (CDKs) that drive cell cycle progression. It does this by dephosphorylating and activating CDKs, which then phosphorylate specific substrates to regulate cell cycle events such as DNA replication and mitosis.

In addition to its role in cell cycle regulation, the recombinant human CDC25C protein has also been shown to have other activities. It has been reported to interact with and regulate the activity of various proteins involved in DNA damage response, apoptosis, and stress response. This highlights the versatility of this protein and its potential role in various cellular processes.

Applications of Recombinant Human CDC25C Protein

The recombinant human CDC25C protein has a wide range of applications in both basic research and drug discovery. Its ability to regulate cell cycle progression makes it a valuable tool for studying the mechanisms of cell division and proliferation. It has also been used to investigate the role of CDC25C in various diseases, including cancer.

Furthermore, the recombinant human CDC25C protein has been utilized in drug discovery efforts targeting the cell cycle. Inhibitors of this protein have been developed as potential anti-cancer therapeutics, as uncontrolled cell cycle progression is a hallmark of cancer cells. The recombinant protein is also used in high-throughput screening assays to identify compounds that modulate its activity.

Antigen Production

The recombinant human CDC25C protein has also been used as an antigen in the development of diagnostic assays and vaccines. It has been shown to induce a strong immune response and can be used to generate antibodies that specifically recognize and bind to the protein. These antibodies can then be used in diagnostic tests to detect the presence of CDC25C in patient samples.

Additionally, the recombinant human CDC25C protein has been used as an antigen in vaccine development against certain types of cancer. By targeting this protein, the vaccine aims to elicit an immune response that specifically targets cancer cells, leading to their destruction.

Conclusion

The recombinant human CDC25C protein is a crucial component of the cell cycle and has a wide range of functions in various cellular processes. Its structure and activity have been extensively studied, and it has numerous applications in both basic research and drug discovery. With its potential role in cancer and other diseases, this protein continues to be an important target for further investigation and development.