Recombinant Human CHMP6 Protein, N-His

Description of Recombinant Human CHMP6 Protein, N-His

Introduction

Recombinant Human CHMP6 Protein, also known as Charged Multivesicular Body Protein 6, is a protein that plays a crucial role in the endosomal sorting complex required for transport (ESCRT) pathway. It is a highly conserved protein that is found in all eukaryotic cells and is involved in various cellular processes such as protein sorting, membrane remodeling, and viral budding. In this article, we will discuss the structure, activity, and applications of Recombinant Human CHMP6 Protein.

Structure of Recombinant Human CHMP6 Protein

Recombinant Human CHMP6 Protein is a 25 kDa protein that consists of 222 amino acids. It is composed of three domains: an N-terminal domain, a central domain, and a C-terminal domain. The N-terminal domain is responsible for protein-protein interactions, while the central domain contains a coiled-coil region that is involved in protein dimerization. The C-terminal domain contains a highly conserved sequence that is essential for the function of CHMP6 in the ESCRT pathway.

The crystal structure of Recombinant Human CHMP6 Protein has been determined, revealing a helical hairpin structure with a V-shaped conformation. This structure is similar to other ESCRT proteins and is believed to be important for the assembly of the ESCRT-III complex. Additionally, the structure of CHMP6 also contains a hydrophobic pocket that is involved in the recognition and binding of specific lipids on the endosomal membrane.

Activity of Recombinant Human CHMP6 Protein

Recombinant Human CHMP6 Protein is a key component of the ESCRT pathway, which is responsible for sorting and trafficking of proteins and lipids in the endosomal system. This pathway is crucial for maintaining proper cellular homeostasis and dysfunction of the ESCRT machinery has been linked to various diseases such as neurodegeneration and cancer.

CHMP6 interacts with other ESCRT proteins to form the ESCRT-III complex, which is responsible for the final scission of the endosomal membrane and the release of intraluminal vesicles (ILVs) into the lumen of multivesicular bodies (MVBs). These ILVs contain cargo proteins that are targeted for degradation in the lysosome or are involved in intercellular communication through exosomes.

In addition to its role in the ESCRT pathway, Recombinant Human CHMP6 Protein has also been shown to play a role in cytokinesis, the process of cell division. It localizes to the midbody during cytokinesis and is involved in the final abscission of the daughter cells.

Applications of Recombinant Human CHMP6 Protein

Recombinant Human CHMP6 Protein has various applications in both research and therapeutic fields. One of the major applications is in the study of the ESCRT pathway and its role in various cellular processes. Recombinant CHMP6 can be used to study the structure and function of the ESCRT-III complex and its interactions with other ESCRT proteins.

Furthermore, Recombinant Human CHMP6 Protein has potential therapeutic applications. As mentioned earlier, dysfunction of the ESCRT machinery has been linked to various diseases. Therefore, targeting CHMP6 or other ESCRT proteins could provide a potential treatment for these diseases. Additionally, CHMP6 has also been shown to play a role in viral budding, making it a potential target for antiviral therapies.

Conclusion

In conclusion, Recombinant Human CHMP6 Protein is a crucial component of the ESCRT pathway and plays a vital role in various cellular processes. Its structure, activity, and applications have been extensively studied and continue to be a topic of interest in the scientific community. With its potential therapeutic applications, CHMP6 holds promise for the development of new treatments for various diseases.