Recombinant Human ID3 Protein, N-His-SUMO

Description of Recombinant Human ID3 Protein, N-His-SUMO

Introduction

Recombinant proteins have revolutionized the field of biotechnology, providing a means to produce large quantities of specific proteins for research, diagnostic, and therapeutic purposes. One such protein is Recombinant Human ID3 Protein, a member of the inhibitor of differentiation (ID) family. In this article, we will explore the structure, activity, and application of this important recombinant protein.

Structure of Recombinant Human ID3 Protein

Recombinant Human ID3 Protein is a 17 kDa protein composed of 152 amino acids. It belongs to the ID family, which consists of four members (ID1-4) that play a crucial role in cell differentiation and proliferation. The ID proteins lack a DNA-binding domain, but they can inhibit the activity of basic helix-loop-helix (bHLH) transcription factors by forming inactive heterodimers with them.

The structure of Recombinant Human ID3 Protein is characterized by a highly conserved helix-loop-helix domain, which is responsible for its interaction with bHLH transcription factors. This domain is flanked by N- and C-terminal regions that are less conserved among the ID family members. The N-terminal region is thought to be involved in protein-protein interactions, while the C-terminal region may play a role in regulating the stability of the protein.

Activity of Recombinant Human ID3 Protein

Recombinant Human ID3 Protein is a potent inhibitor of cell differentiation and proliferation. It exerts its activity by binding to bHLH transcription factors and preventing them from binding to DNA and activating gene expression. This inhibition of bHLH transcription factors is crucial for maintaining the undifferentiated state of stem cells and promoting cell proliferation in various tissues.

In addition to its role in regulating cell differentiation and proliferation, Recombinant Human ID3 Protein has been shown to have anti-apoptotic effects, protecting cells from programmed cell death. It has also been implicated in promoting angiogenesis, the formation of new blood vessels, which is essential for tissue growth and repair.

Application of Recombinant Human ID3 Protein

Recombinant Human ID3 Protein has a wide range of applications in both research and medicine. In research, it is commonly used as a tool to study the role of ID proteins in cell differentiation and proliferation. Its ability to inhibit bHLH transcription factors makes it a valuable tool for investigating the downstream effects of these factors on gene expression.

In medicine, Recombinant Human ID3 Protein has potential therapeutic applications. Its ability to promote cell proliferation and angiogenesis makes it a promising candidate for tissue regeneration and wound healing. It has also been shown to have potential anti-cancer effects, as it can inhibit the growth of certain types of cancer cells by preventing their differentiation and promoting their survival.

Furthermore, Recombinant Human ID3 Protein has been used as an antigen in diagnostic tests for various diseases. Its ability to induce an immune response makes it a useful tool for detecting the presence of specific antibodies in patient samples, aiding in the diagnosis of diseases such as cancer and autoimmune disorders.

Conclusion

In summary, Recombinant Human ID3 Protein is a 17 kDa protein with a highly conserved helix-loop-helix domain and is a member of the ID family. It exerts its activity by inhibiting bHLH transcription factors, and has important roles in cell differentiation, proliferation, and survival. Its wide range of applications in research and medicine make it a valuable tool for understanding the complex processes of cell regulation and for developing potential therapeutics and diagnostic tests.