Recombinant Human HAX1 Protein, N-GST & C-His

Structure of Recombinant Human HAX1 Protein

Recombinant Human HAX1 Protein, also known as HCLS1-associated protein X-1, is a 12-kDa protein that is encoded by the HAX1 gene located on chromosome 1. It is composed of 114 amino acids and has a predicted molecular weight of approximately 12.5 kDa. The protein is highly conserved in mammals, with 99% sequence identity between human and mouse HAX1 proteins.

The structure of Recombinant Human HAX1 Protein consists of a single domain with three alpha helices and a beta strand. These secondary structures are stabilized by four disulfide bonds, which are essential for the protein’s stability and function. The protein also contains a proline-rich region, which is involved in protein-protein interactions.

Recombinant Human HAX1 Protein is produced using recombinant DNA technology, where the HAX1 gene is inserted into a host cell, such as E. coli, and expressed to produce the protein in large quantities. The recombinant protein is then purified using various chromatography techniques to obtain a highly pure and functional protein.

Activity of Recombinant Human HAX1 Protein

Recombinant Human HAX1 Protein plays a crucial role in various cellular processes, including cell survival, proliferation, and apoptosis. It is a member of the Bcl-2 family of proteins and functions as an anti-apoptotic protein by inhibiting the activation of caspases, which are enzymes involved in cell death.

Additionally, Recombinant Human HAX1 Protein is involved in the regulation of mitochondrial membrane potential and the maintenance of mitochondrial morphology. It interacts with several proteins, including HCLS1, which is involved in T-cell signaling, and VDAC1, which is a mitochondrial outer membrane protein. These interactions suggest that Recombinant Human HAX1 Protein may also play a role in immune system function and cellular energy metabolism.

Moreover, studies have shown that Recombinant Human HAX1 Protein is essential for the survival of hematopoietic stem cells and plays a critical role in the development and function of various blood cells, including T-cells, B-cells, and natural killer cells. It is also involved in the regulation of cell adhesion and migration, suggesting a potential role in cancer metastasis.

Application of Recombinant Human HAX1 Protein

Due to its anti-apoptotic and cell survival properties, Recombinant Human HAX1 Protein has potential applications in various fields, including cancer research, immunology, and regenerative medicine.

In cancer research, Recombinant Human HAX1 Protein can be used to study the mechanisms of cell survival and apoptosis, which are essential processes in cancer development and progression. It can also be used as a potential target for cancer therapy, as inhibiting its activity may induce cell death in cancer cells.

In immunology, Recombinant Human HAX1 Protein can be used to study the role of Bcl-2 family proteins in immune cell function and regulation. It can also be used to investigate the role of HAX1 in T-cell signaling and its potential involvement in immune-related diseases.

Furthermore, Recombinant Human HAX1 Protein has potential applications in regenerative medicine, particularly in the field of hematopoietic stem cell transplantation. It can be used to enhance the survival and function of transplanted stem cells, leading to improved outcomes in patients with blood disorders or immune deficiencies.

Conclusion

In summary, Recombinant Human HAX1 Protein is a 12-kDa protein that plays a critical role in cell survival,