Recombinant Human JPH3/JP-3 Protein, N-His

Description of Recombinant Human JPH3/JP-3 Protein, N-His

Introduction to Recombinant Human JPH3/JP-3 Protein

Recombinant Human JPH3/JP-3 Protein, also known as junctophilin-3, is a protein that plays a crucial role in maintaining the structure and function of the cardiac muscle. It is a member of the junctophilin protein family and is primarily found in the junctional complexes of the heart, where it helps in the formation of the excitation-contraction coupling machinery. In this article, we will discuss the structure, activity, and applications of Recombinant Human JPH3/JP-3 Protein.

Structure of Recombinant Human JPH3/JP-3 Protein

The human JPH3/JP-3 gene is located on chromosome 16 and encodes for a protein of 696 amino acids. The protein has a predicted molecular weight of approximately 80 kDa and is composed of four major domains – a C-terminal transmembrane domain, a central coiled-coil domain, and two N-terminal amphipathic helix domains. The coiled-coil domain is responsible for the interaction of JPH3/JP-3 with other proteins, while the amphipathic helix domains are involved in the anchoring of the protein to the cell membrane.

Activity of Recombinant Human JPH3/JP-3 Protein

Recombinant Human JPH3/JP-3 Protein has been shown to play a crucial role in the formation and maintenance of the junctional complexes in the heart. These complexes are essential for the proper functioning of the cardiac muscle, as they facilitate the communication between the cell membrane and the sarcoplasmic reticulum. JPH3/JP-3 acts as a bridge between the two organelles, bringing them in close proximity and allowing for efficient calcium signaling, which is necessary for muscle contraction.

Moreover, JPH3/JP-3 has been found to interact with other proteins involved in the excitation-contraction coupling machinery, such as ryanodine receptors, voltage-gated calcium channels, and calsequestrin. These interactions are crucial for the proper functioning of the cardiac muscle and help in maintaining the balance of calcium ions within the cell.

Applications of Recombinant Human JPH3/JP-3 Protein

The primary application of Recombinant Human JPH3/JP-3 Protein is in the study of cardiac muscle function and disorders. Mutations in the JPH3/JP-3 gene have been linked to the development of hypertrophic cardiomyopathy, a condition characterized by thickening of the heart muscle, leading to impaired heart function. Recombinant Human JPH3/JP-3 Protein can be used to study the effects of these mutations on the structure and activity of the protein and its role in the development of the disease.

In addition, JPH3/JP-3 has also been found to be dysregulated in other cardiac disorders, such as heart failure and arrhythmias. Recombinant Human JPH3/JP-3 Protein can be used to investigate the role of JPH3/JP-3 in these conditions and potentially identify it as a therapeutic target.

Furthermore, Recombinant Human JPH3/JP-3 Protein has also been studied in the context of skeletal muscle function. It has been shown to play a role in the formation of the triad junctions, which are essential for the excitation-contraction coupling in skeletal muscle. Therefore, JPH3/JP-3 may have potential applications in the study of skeletal muscle disorders as well.

Conclusion

In conclusion, Recombinant Human JPH3/JP-3 Protein is a crucial protein involved in the formation and maintenance of junctional complexes in the heart. Its interactions with other proteins in the excitation-contraction coupling machinery make it essential for proper cardiac muscle function. The study of Recombinant Human JPH3/JP-3 Protein has potential applications in understanding and treating various cardiac and skeletal