Recombinant Mouse S100A8 Protein, N-His-SUMO

Description of Recombinant Mouse S100A8 Protein, N-His-SUMO

Introduction

Recombinant Mouse S100A8 Protein is a synthetic form of the S100A8 protein that is produced through the process of recombinant DNA technology. This protein is a member of the S100 family of calcium-binding proteins and is involved in various cellular processes such as inflammation, immune response, and cell differentiation. In this article, we will discuss the structure, activity, and applications of this important protein.

Structure

The recombinant mouse S100A8 protein is made up of 93 amino acids with a molecular weight of approximately 10 kDa. It contains two calcium-binding EF-hand motifs and forms a homodimer in its active form. The crystal structure of this protein has been extensively studied and it has been found that the dimerization of S100A8 is crucial for its biological activity.

Activity

S100A8 protein is known to play a crucial role in inflammation and immune response. It is primarily expressed by neutrophils and monocytes and is involved in the recruitment and activation of immune cells at the site of inflammation. The calcium-binding activity of S100A8 is essential for its interaction with target proteins and its subsequent biological effects. It has been shown to bind to various receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation end products (RAGE), leading to the activation of downstream signaling pathways.

Applications

Recombinant Protein Production

Recombinant mouse S100A8 protein is widely used in the production of recombinant proteins. It is often used as a fusion partner with other proteins to improve their solubility and stability. The calcium-binding activity of S100A8 also helps in the purification of recombinant proteins through affinity chromatography.

Antigen in Immunological Studies

S100A8 protein has been identified as an important antigen in various immunological studies. It is known to induce an immune response and is often used as a target in diagnostic assays for autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Recombinant S100A8 protein is also used in the development of vaccines and immunotherapies for various diseases.

Inflammation Research

Due to its role in inflammation, recombinant mouse S100A8 protein is widely used in research studies related to inflammatory diseases. It is often used to induce inflammation in animal models and to study the mechanisms involved in the inflammatory response. The use of recombinant S100A8 protein has also provided insights into potential therapeutic targets for inflammatory conditions.

Cell Differentiation Studies

S100A8 protein has been shown to play a role in cell differentiation and is involved in the regulation of various cellular processes such as cell growth and migration. Recombinant S100A8 protein is used in cell culture studies to understand its role in cell differentiation and its potential implications in cancer and other diseases.

Diagnostic Applications

Recombinant S100A8 protein is also used in diagnostic applications such as ELISA and western blotting to detect its presence in various biological samples. Its use as a biomarker has been explored in various diseases such as sepsis, inflammatory bowel disease, and cancer.

Therapeutic Applications

The potential therapeutic applications of recombinant mouse S100A8 protein are currently being investigated. It has been shown to have anti-inflammatory properties and has been proposed as a potential treatment for inflammatory diseases. Its