Recombinant Mouse TGM2 Protein, N-His

Description of Recombinant Mouse TGM2 Protein, N-His

Introduction to Recombinant Mouse TGM2 Protein

Recombinant proteins are artificially produced proteins that are derived from genetic material and expressed in host cells. These proteins have a wide range of applications in biotechnology, medicine, and research. One such recombinant protein is the Recombinant Mouse TGM2 Protein.

TGM2, also known as Transglutaminase 2, is a multifunctional enzyme that plays a crucial role in various biological processes, including cell adhesion, apoptosis, and inflammation. It is a calcium-dependent enzyme that catalyzes the formation of covalent bonds between proteins or peptides, resulting in their crosslinking. TGM2 is expressed in various tissues, including the skin, liver, and brain.

Structure of Recombinant Mouse TGM2 Protein

The Recombinant Mouse TGM2 Protein is a 78 kDa protein that consists of 687 amino acids. It has a highly conserved domain known as the catalytic core, which is responsible for its enzymatic activity. This domain contains the active site, which binds to calcium ions and substrates, and the crosslinking site, which catalyzes the crosslinking reaction.

The protein also has a N-terminal β-sandwich domain, which is involved in substrate recognition and binding. It also contains a C-terminal domain, which is responsible for its localization and interaction with other proteins.

Activity of Recombinant Mouse TGM2 Protein

The primary function of TGM2 is to catalyze the formation of ε-(γ-glutamyl) lysine isopeptide bonds between proteins or peptides. This crosslinking activity is essential for maintaining the structural integrity of tissues and promoting cell adhesion. TGM2 also has transamidation activity, which involves transferring the acyl group from one substrate to another.

Apart from its enzymatic activity, TGM2 also has non-enzymatic functions, such as regulating cell signaling and apoptosis. It can interact with various proteins and modulate their activity, thereby affecting various cellular processes.

Application of Recombinant Mouse TGM2 Protein

The Recombinant Mouse TGM2 Protein has numerous applications in biotechnology, medicine, and research. One of its main applications is in the production of recombinant proteins. TGM2 can be used to crosslink proteins, thereby increasing their stability and half-life. This is particularly useful in the production of therapeutic proteins, such as antibodies, which require high stability and prolonged activity.

In medicine, TGM2 has been implicated in various diseases, such as celiac disease, neurodegenerative disorders, and cancer. Therefore, the Recombinant Mouse TGM2 Protein can be used as an antigen in diagnostic tests for these diseases. It can also be used to study the role of TGM2 in disease progression and as a potential therapeutic target.

In research, TGM2 is widely used in studies involving cell adhesion, apoptosis, and inflammation. The Recombinant Mouse TGM2 Protein can be used to study the mechanisms of these processes and to develop new treatments for related diseases.

Conclusion

In conclusion, the Recombinant Mouse TGM2 Protein is a versatile protein with various functions and applications. Its structure, enzymatic activity, and non-enzymatic functions make it a valuable tool in biotechnology, medicine, and research. As our understanding of TGM2 continues to grow, so does its potential for further applications in various fields.