Recombinant Human ACTN1 Protein, N-His

Description of Recombinant Human ACTN1 Protein, N-His

Introduction to Recombinant Human ACTN1 Protein

Recombinant Human ACTN1 protein, also known as alpha-actinin-1, is a cytoskeletal protein that plays a crucial role in maintaining the structural integrity of cells. It is encoded by the ACTN1 gene and is primarily found in non-muscle cells, such as fibroblasts, endothelial cells, and epithelial cells. This protein has been extensively studied due to its involvement in various cellular processes, including cell adhesion, migration, and signaling. In this article, we will explore the structure, activity, and applications of recombinant Human ACTN1 protein.

Structure of Recombinant Human ACTN1 Protein

Recombinant Human ACTN1 protein is a member of the spectrin superfamily and is composed of 894 amino acids. It consists of three major domains: an N-terminal actin-binding domain (ABD), a central rod domain, and a C-terminal calmodulin-like domain (CaM-LD). The ABD contains two calponin homology (CH) domains, which are responsible for binding to actin filaments. The rod domain is composed of spectrin repeats, which are responsible for dimerization and binding to other proteins. The CaM-LD is responsible for binding to calcium ions and regulating the activity of the protein.

Activity of Recombinant Human ACTN1 Protein

Recombinant Human ACTN1 protein is involved in various cellular activities, including cell adhesion, migration, and signaling. It plays a crucial role in maintaining the structural integrity of cells by cross-linking actin filaments and connecting them to the plasma membrane. This allows cells to withstand mechanical stress and maintain their shape. In addition, ACTN1 is involved in cell migration by regulating the formation and turnover of focal adhesions, which are essential for cell movement. Furthermore, ACTN1 has been shown to play a role in cell signaling by interacting with various signaling molecules, such as integrins and growth factor receptors.

Applications of Recombinant Human ACTN1 Protein

Recombinant Human ACTN1 protein has various applications in both research and therapeutic settings. In research, it is commonly used as an antigen in studies related to cytoskeletal dynamics, cell adhesion, and migration. It is also used as a tool to study the role of ACTN1 in diseases, such as cancer and cardiovascular disorders. In addition, recombinant Human ACTN1 protein is used in protein-protein interaction studies to identify its binding partners and understand its role in different signaling pathways.

In therapeutic settings, recombinant Human ACTN1 protein has potential applications in tissue engineering and regenerative medicine. Its ability to cross-link actin filaments and connect them to the plasma membrane makes it a promising candidate for promoting cell adhesion and tissue formation. Furthermore, ACTN1 has been shown to play a role in wound healing, making it a potential target for developing therapies for chronic wounds.

Conclusion

Recombinant Human ACTN1 protein is a crucial cytoskeletal protein that is involved in various cellular processes. Its structure, consisting of an actin-binding domain, a rod domain, and a calmodulin-like domain, allows it to perform its functions of maintaining cell structure, regulating cell migration, and participating in cell signaling. This protein has diverse applications in research and therapeutics, making it a valuable tool for understanding cellular processes and developing potential treatments for various diseases. Overall, recombinant Human ACTN1 protein is an essential protein that continues to be extensively studied for its role in maintaining the structural integrity of cells.