Recombinant Human JADE1 Protein, N-His

Description of Recombinant Human JADE1 Protein, N-His

The Structure and Function of Recombinant Human JADE1 Protein

Recombinant proteins are proteins that are engineered in a laboratory by combining DNA sequences from different sources. These proteins have become an essential tool in the field of biotechnology and have a wide range of applications in medicine, agriculture, and research. One such recombinant protein is the human JADE1 protein, which plays a crucial role in regulating gene expression and has potential therapeutic applications.

Structure of Recombinant Human JADE1 Protein

The human JADE1 gene is located on chromosome 19 and encodes for a protein of 345 amino acids. The recombinant human JADE1 protein is produced by cloning the DNA sequence of the JADE1 gene into a suitable expression vector and expressing it in a host cell, typically E. coli or mammalian cells. The resulting protein has a molecular weight of approximately 38 kDa.

The JADE1 protein belongs to the Jade family of proteins, which also includes JADE2 and JADE3. These proteins share a conserved domain known as the Jade domain, which is responsible for their interaction with other proteins. The Jade domain is located at the C-terminus of the JADE1 protein and is essential for its function.

Function of Recombinant Human JADE1 Protein

The primary function of JADE1 protein is to regulate gene expression by acting as a transcriptional co-repressor. It does so by interacting with other proteins, such as histone deacetylases (HDACs) and histone methyltransferases (HMTs), which modify chromatin structure and regulate gene expression. JADE1 has been shown to play a crucial role in embryonic development, cell proliferation, and differentiation.

Additionally, JADE1 has been found to be involved in various cellular processes, including DNA damage response, cell cycle progression, and apoptosis. It has been shown to interact with several tumor suppressor proteins, suggesting its potential role in cancer development and progression.

Application of Recombinant Human JADE1 Protein

The recombinant human JADE1 protein has various potential applications in both research and medicine. One of the significant research applications of JADE1 is in the study of gene regulation and epigenetics. Its role as a transcriptional co-repressor makes it a valuable tool in understanding the mechanisms of gene expression and its dysregulation in diseases.

In terms of therapeutic applications, JADE1 has shown promise in cancer treatment. Studies have shown that JADE1 expression is reduced in various types of cancer, and its overexpression can inhibit tumor growth and induce cell death. This makes JADE1 a potential target for cancer therapy, and its recombinant form could be used for targeted drug delivery.

Moreover, JADE1 has also been implicated in several other diseases, such as neurodevelopmental disorders and cardiovascular diseases. Its recombinant form could be used for diagnostic purposes and as a potential therapeutic agent in these conditions.

Conclusion

In summary, the recombinant human JADE1 protein is a crucial player in regulating gene expression and has potential applications in both research and medicine. Its structure, function, and potential applications make it a valuable tool in understanding and treating various diseases. Further research and development of JADE1 could lead to the development of novel therapies for various disorders, making it an exciting area of study in the field of biotechnology.