Recombinant Human MSH6 Protein, N-His

Description of Recombinant Human MSH6 Protein, N-His

Introduction

Recombinant Human MSH6 Protein, also known as MutS homolog 6, is a protein that plays a crucial role in DNA mismatch repair. This protein is encoded by the MSH6 gene and is a member of the MutS family of proteins. Recombinant Human MSH6 Protein is widely used in various research and diagnostic applications due to its unique structure and activity. In this article, we will discuss the structure, activity, and applications of Recombinant Human MSH6 Protein.

Structure of Recombinant Human MSH6 Protein

Recombinant Human MSH6 Protein is a 136 kDa protein with a total of 1362 amino acids. It is composed of two domains, the N-terminal domain and the C-terminal domain. The N-terminal domain contains the ATP-binding site, while the C-terminal domain contains the mismatch recognition site. The two domains are connected by a linker region, which is responsible for the communication between the two domains. The crystal structure of Recombinant Human MSH6 Protein has been determined, providing insights into the protein’s function and mechanism of action.

Activity of Recombinant Human MSH6 Protein

Recombinant Human MSH6 Protein is a key component of the DNA mismatch repair system. It recognizes and binds to DNA mismatches, which are errors in the DNA sequence that occur during DNA replication or due to environmental factors. Once bound, Recombinant Human MSH6 Protein recruits other proteins to form a complex that repairs the mismatched DNA. This process is crucial for maintaining the integrity of the genome and preventing the accumulation of mutations that can lead to diseases such as cancer.

In addition to its role in DNA mismatch repair, Recombinant Human MSH6 Protein also plays a role in DNA damage response and cell cycle regulation. It has been shown to interact with other proteins involved in these processes, further highlighting its diverse functions in the cell.

Applications of Recombinant Human MSH6 Protein

Recombinant Human MSH6 Protein has numerous applications in the field of research and diagnostics. Some of the key applications are:

DNA Mismatch Repair Studies

Recombinant Human MSH6 Protein is widely used in studies related to DNA mismatch repair. Its ability to recognize and bind to DNA mismatches makes it a valuable tool for understanding the mechanisms and regulation of this process. It is often used in combination with other proteins involved in DNA mismatch repair to study their interactions and functions.

Cancer Research

Mutations in the MSH6 gene have been linked to hereditary nonpolyposis colorectal cancer (HNPCC), also known as Lynch syndrome. Recombinant Human MSH6 Protein is used in cancer research to study the role of MSH6 mutations in the development and progression of cancer. It is also used to develop diagnostic tests for identifying individuals with Lynch syndrome.

Diagnostics

Recombinant Human MSH6 Protein is used in diagnostic tests to detect mutations in the MSH6 gene. These tests are important for identifying individuals with Lynch syndrome and other hereditary cancer syndromes. They can also be used to predict the response of certain cancers to specific treatments.

Drug Development

The DNA mismatch repair system and Recombinant Human MSH6 Protein have been identified as potential targets for cancer therapy. Therefore, Recombinant Human MSH6 Protein is used in drug development studies to identify compounds that can modulate its activity and potentially be used as cancer treatments.

Conclusion

In summary, Recombinant Human MSH6 Protein is a crucial protein involved in DNA mismatch repair, DNA damage response, and cell cycle regulation. Its unique structure and activity make it a valuable tool in various research and diagnostic applications. With the increasing understanding of its functions, Recombinant Human MSH6 Protein is expected to play an even more significant role in the development of new treatments for cancer and other diseases.