Recombinant Human PRMT1 Protein, N-His

Description of Recombinant Human PRMT1 Protein, N-His

Introduction to Recombinant Human PRMT1 Protein

Recombinant Human PRMT1 Protein, also known as Protein Arginine Methyltransferase 1, is a key enzyme involved in the process of protein methylation. This protein is produced through recombinant DNA technology, where the gene for PRMT1 is inserted into a host organism, typically bacteria, to produce large quantities of the protein for research and therapeutic purposes.

Structure of Recombinant Human PRMT1 Protein

The Recombinant Human PRMT1 Protein is a 353 amino acid protein with a molecular weight of approximately 40 kDa. It contains a catalytic domain responsible for its methyltransferase activity, as well as a SAM (S-adenosylmethionine) binding site and a zinc finger domain. The protein also has several conserved motifs, including the double E loop and the F/Y-rich loop, which are crucial for its enzymatic function.

Activity of Recombinant Human PRMT1 Protein

Recombinant Human PRMT1 Protein is a type I protein arginine methyltransferase, which means it catalyzes the transfer of a methyl group from SAM to arginine residues in target proteins. This methylation process plays a crucial role in regulating various cellular processes, including gene expression, protein-protein interactions, and signal transduction pathways.

PRMT1 specifically targets arginine residues located within glycine-arginine-rich (GAR) motifs, and catalyzes the formation of monomethylarginine (MMA) and asymmetric dimethylarginine (ADMA) on these residues. These modifications can have significant effects on the structure and function of target proteins, such as altering their stability, activity, and interactions with other molecules.

Applications of Recombinant Human PRMT1 Protein

Due to its crucial role in protein methylation, Recombinant Human PRMT1 Protein has a wide range of applications in both research and therapeutic fields.

Research Applications

Recombinant Human PRMT1 Protein is commonly used in research to study the effects of protein methylation on various cellular processes. It can be used to investigate the role of PRMT1 in specific pathways, as well as to identify and characterize its target proteins. Additionally, the protein can be used to study the effects of PRMT1 inhibitors and activators on cellular processes, providing valuable insights into potential therapeutic targets.

Therapeutic Applications

Due to its involvement in various cellular processes, Recombinant Human PRMT1 Protein has also shown potential as a therapeutic target for various diseases. For example, PRMT1 has been linked to cancer development and progression, making it a potential target for cancer treatment. Inhibitors of PRMT1 have also shown promise in treating neurodegenerative diseases, such as Alzheimer’s and Parkinson’s disease.

Furthermore, Recombinant Human PRMT1 Protein has been used in the development of diagnostic tools for diseases that involve PRMT1 dysregulation. For instance, ADMA, the product of PRMT1 activity, has been found to be elevated in patients with cardiovascular diseases, making it a potential biomarker for early diagnosis and monitoring of these conditions.

Conclusion

Recombinant Human PRMT1 Protein is a crucial enzyme involved in protein methylation, with a wide range of applications in research and therapeutics. Its structure, activity, and role in various cellular processes make it a valuable tool for understanding the complex mechanisms of protein regulation and identifying potential therapeutic targets. As research in this field continues to advance, Recombinant Human PRMT1 Protein will undoubtedly play a crucial role in furthering our understanding of protein methylation and its implications in health and disease.