XtenCHO™ Race, the new mammalian transient expression system

Based on our expertise:

• The XtenCHO system is generally better suited for antibody production, with higher transient production yields observed compared to HEK293.
• The HEK293 system is often more appropriate for intracellular protein production.

Additionally, it’s important to note that the majority of FDA-approved antibody-based drugs are produced using CHO expression systems, highlighting their reliability and effectiveness for antibody-related applications.

Here are a few publications and a patent that showcase how some of our customers have utilized XtenCHO :
https://patents.google.com/patent/WO2024194298A1/en
https://doi.org/10.3389/fimmu.2022.848571
https://doi.org/10.4049/jimmunol.2100595
https://doi.org/10.1186/s13195-022-01141-1
https://doi.org/10.1038/s41467-022-35700-9

XtenCHO™ Race cells perform exceptionally well across various culture scales and are particularly efficient in low-volume productions. They can be used in formats such as 24-well deep-well plates, 50 mL vented flasks, 3 L flasks, and even in bioreactors exceeding 10 L.

We provide an optimized protocol with recommended volumes tailored to different culture sizes to ensure optimal performance.

The XtenCHO™ Expression Medium is serum-free, animal-component-free, and chemically defined. It requires additional supplementation with L-Glutamine and an anti-clumping agent both before and after transfection to ensure optimal performance.

After purchasing the starter kit, you’ll need to reorder consumables such as the XtenCHO™ Expression Medium, L-Glutamine, and anti-clumping agent as needed to continue your experiments.

The Starter Kit provides enough materials for 1L of production. Once used, you can reorder the following components for additional 1L productions:

• XtenCHO™ Expression Medium (PX-XTE-002)
• XtenCHO™ Transfection Kit (PX-XTE-R-003)

Yes, we do. We maintain a stock of all the components for our kits, ensuring worldwide delivery within 2 to 3 days.

Each item is stable for 12 months from the date of manufacture if the cells, reagents, and medium are stored at their optimal storage temperatures. Diluted XtenFect Reagent, the working solution, and XtenCHO™ Race Enhancer should be used within 4 weeks after dilution or thawing.

We recommend using the pXten1 Expression Vector for expressing your protein of interest. While XtenCHO™ Race Cells support high expression levels from various expression plasmids, they perform best when pXten1 is used as the expression vector. The chosen vector must include the CMV promoter.

Our XtenCHO™ Race Cells are derived from CHO-K1 cells, making them compatible with standard CHO-K1 selection methods. However, please note that XtenCHO™ Race Cells are engineered to be resistant to geneticin. We recommend using an alternative selection method to establish stable cell lines.

Yes, it is possible to adapt your CHO cells to the XtenCHO™ Expression Medium. This adaptation may enhance the productivity of your CHO cells and support high-density growth. However, there is no guarantee that other CHO cell lines will achieve the same expression levels as the XtenCHO™ Race Cells. XtenCHO™ Race Cells have been specifically engineered for optimal protein expression. When combined with the pXten1 Expression Vector, the optimized protocol, and reagents, these cells enable extended transient gene expression by enhancing plasmid-driven expression and improving plasmid maintenance.

The XtenCHO™ Race Starter Kit arrives precisely when you need it, keeping your projects on schedule and ensuring smooth, uninterrupted productivity. Good to know : Free shipping in EU for orders>500€

Europe: 2-4 business days
USA & Canada: 2-4 business days
Rest of the world: 5-10 business days