ProteoGenix can generate high-diversity antibody libraries for phage display providing you with the most refined binders possible for your project. The construction of an immune library potentially increases the affinity of your final antibody against the targeted protein.

An immune library requires prior immunization, which
takes longer than performing a phage display screening from
a naive library.

  • Any kind of animal species available
  • High-affinity antibodies
CONTACT US

Immune library construction service

ProteoGenix is an expert in antibody production so many antibody libraries from various species can be generated at your convenience: Rabbit, Mouse, Sheep or even Alpaca and Llama (for VHH libraries).

Immunization.

  • 4-6 weeks

Isolation of PBMC:

  • Spleen + Bone marrow.
  • Total RNA extraction.
  • cDNA synthesis
  • 1 week

PCR amplification of antibodies:

  • VH and VL amplification.
  • 1 week

cDNA VH/VL assembling construction of library:

  • phagemid synthesis and cloning.
  • Transformation for production.
  • 1-2 weeks

Total:

  • 7/10 weeks

Advantages of using an immune library for a specific project

Provides binders with a higher affinity compared to a naive library

More antibodies directed against the antigen of interest

Ideal for small and modified antigens (specific antibody with low capacity)

Allows work with any species as there is no fusion necessary compared to hybridoma technique

(example for rabbit monoclonal antibodies)

CONTACT US

Construction and screening of an immune library

The library capacity is measured at 109 to 1016 different binders. We are able to offer you several types of libraries: scFv, Fab and VHH. The library screening is performed to identify binders with the best affinity.
Construction and screening of an immune library can be tailored to your research for high specificity and stability.

The screening process is based on our standard protocol of panning/biopanning and ProteoGenix can adapt this protocol to any request. You can find out more about the stages of biopanning in the screening from a naive library.

After 3 to 5 rounds of biopanning, ProteoGenix provides you with several binders (3 to 10) whilst giving you the option to ask for more if required.

Example of panning (biopanning) protocol
Example of panning (biopanning) protocol in Phage display. 1 - Incubation with phage library 2 - Wash away non-binders 3 - Elution of binders 4 - 3 to 5 rounds of panning/biopanning process 5 - ELISA to determine specificity of binding after 3-5 rounds of biopanning

At ProteoGenix, the choice of building and screening an immune library by phage display will allow you to produce monoclonal antibodies with the highest affinity for any species.

For more information or to get a quote for our phage display services with the use of immune libraries, don’t hesitate to contact us.

CONTACT US

Related sections which could interest you: