Do you need an antibody that isn’t commercially available to characterize a new target, or are you dissatisfied with the one you’re currently using? With ProteoGenix’s custom monoclonal antibodies, you can obtain a tailor-made antibody fully validated in the application of your choice.

What does our custom monoclonal antibody development service include?

Core services

Our custom monoclonal antibody development service includes antigen design and production from the sequence provided, antibody generation using one of our three proprietary platforms, as well as antibody production and purification.

Additional services

This service can also be supplemented with optional steps such as conjugation to peroxidase or fluorochromes, or conversion for recombinant production (transient or stable production).

Our customized monoclonal antibody development process in detail

Antigen design and production

As experts in peptide and protein production, ProteoGenix can utilize a wide range of macromolecules as antigens, including:

  • Soluble or membrane-bound proteins in their native conformation
  • Modified or unmodified peptides
  • Haptens
  • Cells
  • Nucleic acids…
Method Turnaround Time (may vary depending on the project)
Phage Display – Naïve Library From 4 weeks
Phage Display – Immune Library From 10 weeks
Single B cell screening From 7 weeks
Hybridoma generation From 9 weeks
Method Application
Hybridoma cell culture Small-scale production
Mouse ascites production Small-scale production
High-throughput antibody production Parallel testing of dozens or hundreds of sequences
Transient antibody production Small-scale production
Stable cell line generation Large-scale and long-term production (manufacturing)

Antibody conjugation

If needed, our teams can conjugate the produced antibodies with various peroxidases or fluorochromes to suit your application, whether for flow cytometry, detection assays, or other uses.

Interested in learning more? Feel free to contact our experts by clicking here.

What means “Application guaranteed Custom Monoclonal Antibodies”?

We work for your success. Your satisfaction with the antibodies we produce is our top priority. For this reason, we offer “application guaranteed” packages for research and diagnostic applications, ensuring optimal performance in your specific application.

Here’s how it works:

  1. We produce the antibodies.
  2. You receive samples to validate in your application.
  3. We initiate production of the validated antibodies you’ve selected.

We guarantee performance in the following applications:

ELISA
Sandwich ELISA
Western Blot
Flow cytometry
Immuno fluorescence
Immuno precipitation
Immuno histochemistry

This is the best way we have found to ensure you receive an antibody that perfectly meets your needs.

Case Studies

Our diverse antibody development platforms allow us to deliver custom monoclonal antibodies with guaranteed performance in your chosen application. To illustrate our process, here are two case studies where you’ll find:

    • Project description
    • Strategies used for antibody generation
    • Project-specific results

Please note, our guarantees consider both results obtained by ProteoGenix and successful outcomes of supplied antibodies in your own lab setting.

  • Case Report

    Generation of an Antibody Validated in Flow Cytometry

    Download now

  • Case Report

    Development of a Sandwich ELISA

    Download now

Testimonial

“We are thrilled with the success we’ve achieved through our collaboration with ProteoGenix in developing monoclonal antibodies targeting our protein. The antibodies they produced were not only highly selective but also demonstrated exceptional affinity for our target, significantly advancing our research. ProteoGenix impressed us with their efficiency and speed throughout the process. Their commitment to providing excellent customer service made our experience even better, as they were always available to address our questions and concerns. We highly recommend ProteoGenix to anyone in need of high-quality antibody development.”

Selected References of Monoclonal Antibodies Developed by ProteoGenix

JP Barnier et al., The minor pilin PilV provides a conserved adhesion site throughout the antigenically variable meningococcal type IV pilus. PNAS 118, (45) e2109364118 (2021).
https://doi.org/10.1073/pnas.2109364118

H Cerutti et al., Large scale production and characterization of SARS-CoV-2 whole antigen for serological test development. J Clin Lab Anal 35, (4) 23735 (2021).
https://doi.org/10.1002/jcla.23735

N Yacov et al., MOSPD2 is a therapeutic target for the treatment of CNS inflammation. Clin Exp Imm 201, (2) 105-20 (2020).
https://doi.org/10.1111/cei.13448

PC Maity et al., IGLV3-21*01 is an inherited risk factor for CLL through the acquisition of a single-point mutation enabling autonomous BCR signaling. PNAS 117, (8) 4320-27 (2020).
https://doi.org/10.1073/pnas.1913810117

M Fresquet et al., Autoantigens PLA2R and THSD7A in membranous nephropathy share a common epitope motif in the N-terminal domain. J Autoimm 106, 102308 (2020).
https://doi.org/10.1016/j.jaut.2019.102308

P Cunha et al., Expansion, isolation and first characterization of bovine Th17 lymphocytes. Nature Sci Rep 9, 16115 (2019).
https://doi.org/10.1038/s41598-019-52562-2

M Krifa et al., Limoniastrum guyonianum aqueous gall extract induces apoptosis in human cervical cancer cells involving p16INK4A re-expression related to UHRF1 and DNMT1 down-regulation. J Exp Clin Canc Res 32, (30) (2013).
https://doi.org/10.1186/1756-9966-32-30

E Faure et al., Probable presence of an ubiquitous cryptic mitochondrial gene on the antisense strand of the cytochrome oxidase I gene. Biol Direct 6, (56) (2011).
https://doi.org/10.1186/1745-6150-6-56

Want to discuss your project?