Most amino acids are encoded by multiple codons that are called synonymous codons. However, specific codons are used more often than others during gene translation.
This phenomenon is called the codon usage bias and is species specific. So, as a result of the redundancy of the genetic code, rare codons can be replaced by codons that are more abundant in the genes of the host organism, without changing the amino acid sequence of the protein, per se.
ProteoGenix’s Codon Optimization Tool leverages a codon bias strategy and additional parameters like GC content, repetitive sequences, avoiding restriction sites and mRNA secondary structure.
Codon optimization makes DNA synthesis easier and improves recombinant protein production yields when expression is done in heterologous hosts.
We therefore strongly recommend to let us optimize the sequence.