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Phage display
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Human
Camelids
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Dog
Mouse
Cat
NEW
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NEW
By format
Fab
scFv
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Anti-MHC-peptide complex antibodies
Anti-GPCR antibodies
Anti virus-like particle antibodies
NEW
Anti-protein/peptide antibodies
Anti-hapten antibodies
Anti-drug antibodies
Antibody engineering services
Antibody Developability
HOT
Humanization
Affinity maturation
Antibody-Drug Conjugates
Bispecific antibodies
CAR-T cell development
Monoclonal antibody characterization
KD measurement
Antibody protein sequencing
Antibody sequencing
Recombinant antibody production
Transient antibody expression
Stable cell lines for monoclonal antibody production
Ascites/hybridoma cell culture
High throughput antibody production
NEW
Polyclonal antibody production
Polyclonal antibody production
Polyclonal Antibody Interactive Form
ELISA assay development
Therapeutic antibody services
Therapeutic antibody services
Fully human antibody development services
CAR-T cell development
Custom antibodies against cancer targets
Custom antibodies against viral targets
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E. coli
B. subtilis
Yeast
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High throughput protein expression
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Peptide Synthesis Interactive Form
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Peptide libraries
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HOT
Overlapping peptide libraries
Truncation peptide libraries
Random peptide libraries
Alanine scanning peptide libraries
Positional scanning peptide libraries
Scrambled peptide libraries
Peptide conjugation and modification
Fluorescent-labeled peptides
Quenched Fluorescent Peptides
Isotope-labeled peptides
Bioreagents
Bioreagents
Antibodies
Antibodies
Primary antibodies
By target
Therapeutic antibodies - Biosimilars
Isotype control antibodies
Anti-Viral proteins
Anti-Immune checkpoint proteins
Nanobodies
Anti-drug antibodies
Tag antibodies
Anti-Interferons
Anti-Biomarker proteins
Anti-Cytokine proteins
Anti-GPCR
Anti-Chemokines & chemokine receptors
Anti-Spike & Anti-RBD Antibodies
Anti-Growth factor proteins
Anti-CD proteins
Anti-Enzymes
Anti-TNF proteins
Anti-Interleukins
Anti-Transcription Factors
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WB
FC
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IP
IF
MS
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Therapeutic Proteins - Biosimilars
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XtenCHO™
XtenCHO™
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IHC guaranteed monoclonal antibody production
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IHC guaranteed monoclonal antibody production
Monoclonal antibody production package details
Package content
Guarantees and price
Deliverables
Lead time
Antigen design (design of the most appropriate fragment to be produced)
Two gene syntheses (each gene optimized for a specific expression system) and two subclonings
Expression tests in two systems taken from
E. coli
,
B. subtilis
,
P. pastoris
, insect cells and mammalian cells. To be determined by ProteoGenix.
Production of the antigen in the most appropriate expression system depending on the yield and the project requirements.
Mice injections: three mice with protein produced in native conditions (if available) and three mice with denatured protein.
Immune response control by ELISA
Selection of the best mice of each three mice batch for two fusions in order to have antibodies targeting the native form of the protein and antibodies targeting the denatured form
Screening of the fusion products by ELISA: around 10 x 96-well plates
Subcloning of two parental clones (2-3 rounds of limiting dilutions) examined by ELISA
Production and purification of 2mg from the best clone
Level 1: If we don’t succeed in producing an antibody that works in ELISA against the immunogen, we charge EUR 1000
Level 2: If we succeed in producing an antibody that works in ELISA against the immunogen, we charge a part of the cost
Level 3: If after testing the purified antibody the customer wants to receive the hybridoma, we charge the full package price
10 -20 best clones at parental stage for customer’s selection
100 µg of purified antibody from the best clone to confirm hybridoma purchase
50 µg of purified protein
After total payment:
1.9 mg of purified antibody
One hybridoma cell line (four frozen vials)
150 µg of purified protein
Both genes in pUC57
Gene synthesis: 3-4 weeks
Protein production : 7-9 weeks
Injections: 8-12 weeks
Fusion & screening:4-5 weeks
Subcloning: 8-12 weeks
Antibody production: 3-4 weeks
Total: 33-46 weeks
Get a free quote
Our application guaranteed monoclonal antibody production packages
Standard ELISA: ATX-PACK1M
Premium ELISA: ATX-PACK2M
Premium WB: ATX-PACK3M
Premium Flow Cytometry: ATX-PACK4M
Premium sandwich ELISA: ATX-PACK5M
Premium IF: ATX-PACK6M
Premium IP: ATX-PACK7M
Peptide: ATX-PACK8M
Modification specific: ATX-PACK9M
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