MousePAD2 Protein-arginine deiminase type-2 Mouse Recombinant Protein

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Product nameMousePAD2 Protein-arginine deiminase type-2 Mouse Recombinant Protein
Uniprot IDQ08642
Uniprot linkhttp://www.uniprot.org/uniprot/Q08642
Expression systemEukaryotic expression
SequenceMGASWSHPQFEKGGGSGGGSGGSAWSHPQFEKMQPPIRENMLRERTVRLQYGSRVEAVYVLGTQLWTDVYSAAPAGAKTFSLKHSEGVKVEVVRDGEAEEVVTNGKQRWALSPSSTLRLSMAQASTEASSDKVTVNYYEEEGSAPIDQAGLFLTAIEISLDVDADRDGEVEKNNPKKASWTWGPEGQGAILLVNCDRDTPWLPKEDCSDEKVYSKQDLQDMSQMILRTKGPDRLPAGYEIVLYISMSDSDKVGVFYVENPFFGQRYIHILGRQKLYHVVKYTGGSAELLFFVEGLCFPDESFSGLVSIHVSLLEYMAEGIPLTPIFTDTVMFRIAPWIMTPNILPPVSVFVCCMKDNYLFLKEVKNLVEKTNCELKVCFQYMNRGDRWIQDEIEFGYIEAPHKGFPVVLDSPRDGNLKDFPIKQLLGPDFGYVTREPLFETVTSLDSFGNLEVSPPVTVNGKEYPLGRILIGSSFPLSGGRRMTKVVRDFLQAQQVQAPVELYSDWLTVGHVDEFMTFIPIPGKKEFRLLMASTSACYQLFREKQKAGHGEAVMFKGLGGMSSKRITINKILSNESLTQENQYFQRCLDWNRDILKRELALTEKDIIDLPALFKMDENHQARAFFPNMVNMIVLDKDLGIPKPFGPQVEEECCLETHVRGLLEPLGLACTFIDDISAYHKFLGEVHCGTNVRRKPFTFKWWHMVP
Molecular weight79.36 kDa
Protein delivered with Tag?Yes
Purity estimated95%
Buffer50mM Tris-Cl pH 8.0, 150mM NaCl
FormLyophilized
Delivery conditionDry Ice
Delivery lead time in business days3-5 days if in stock; 3-5 weeks if production needed
Storage condition4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
BrandProteoGenix
ApplicationsELISA,WB
Fragment TypeFull-length
NCBI ReferenceNP_032838.2
Biological activityCalcium-dependent critrullination activity of the recombinant PAD2 was confirmed with Fibrinogen as substrate (detection of critrullinated fibrinogen by ELISA).
Aliases /SynonymsPAD-H19, PAD2, PDI2, Peptidylarginine deiminase II
ReferencePX-P3022
NoteFor research use only.

Description of MousePAD2 Protein-arginine deiminase type-2 Mouse Recombinant Protein

General Information about Mouse PAD2 (PADI2)

Mouse PAD2 (PADI2) is a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions.

PADI2 has peptidylarginine deiminase activity against synthetic substrates. PAD2 is mainly expressed in the central nervous system, skeletal muscle, spinal cord, cerebrum, cerebellum, and submaxillary gland. Protein arginine deiminase type-2 plays a role in the onset and progression of neurodegenerative human disorders, including Alzheimer disease and multiple sclerosis, and it has also been implicated in glaucoma pathogenesis.

Eukaryote expression systems and more specifically Insect cells are used at ProteoGenix to produce mouse PAD2 recombinant protein with appropriate post translational modifications.

Publication

1. F. Arnoux and al (2017).
Peptidyl arginine deiminase immunization induces anticitrullinated protein antibodies in mice with particular MHC types.
2. Citrullination, a novel posttranslational modification of elastin, is involved in COPD pathogenesis, Mark P. Murphy, Marina Zieger, Michael Henry, Paula Meleady, Christian Mueller, Noel G. McElvaney, and Emer P. Reeves, American Journal of Physiology-Lung Cellular and Molecular Physiology 2024 327:4, L600-L606, doi: 10.1152/ajplung.00185.2024

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