Recombinant Human ADAMTS12 Protein, N-His

Description of Recombinant Human ADAMTS12 Protein, N-His

Introduction to Recombinant Human ADAMTS12 Protein

Recombinant Human ADAMTS12 Protein is a type of protein that is produced using recombinant DNA technology. This protein is a member of the ADAMTS family, which stands for “a disintegrin and metalloproteinase with thrombospondin motifs”. ADAMTS proteins are known to have important roles in various biological processes, including extracellular matrix organization, tissue development, and inflammation. In this article, we will discuss the structure, activity, and application of Recombinant Human ADAMTS12 Protein.

Structure of Recombinant Human ADAMTS12 Protein

The structure of Recombinant Human ADAMTS12 Protein is composed of several domains, including a pro-domain, a metalloproteinase domain, a disintegrin-like domain, a thrombospondin type 1 repeat (TSR) domain, and a C-terminal domain. The pro-domain is responsible for regulating the activation of the protein, while the metalloproteinase domain is responsible for the enzymatic activity of the protein. The disintegrin-like domain is involved in protein-protein interactions, and the TSR domain is responsible for binding to extracellular matrix components. The C-terminal domain is involved in substrate recognition and binding.

The structure of Recombinant Human ADAMTS12 Protein also contains a zinc ion, which is essential for its enzymatic activity. This zinc ion is coordinated by three histidine residues and one cysteine residue in the metalloproteinase domain.

Activity of Recombinant Human ADAMTS12 Protein

Recombinant Human ADAMTS12 Protein is a metalloproteinase, meaning it has the ability to cleave peptide bonds in proteins. Specifically, this protein is a member of the ADAMTS subfamily of metalloproteinases, which are known to have important roles in extracellular matrix remodeling and tissue development.

The activity of Recombinant Human ADAMTS12 Protein is regulated by its pro-domain, which must be removed for the protein to become active. Once activated, this protein can cleave various extracellular matrix components, including aggrecan, versican, and fibronectin. By cleaving these components, Recombinant Human ADAMTS12 Protein is able to modulate the structure and function of the extracellular matrix, which is important for tissue development and repair.

Application of Recombinant Human ADAMTS12 Protein

Recombinant Human ADAMTS12 Protein has various potential applications in both research and clinical settings. One of the main applications of this protein is in studying its role in tissue development and repair. By understanding how Recombinant Human ADAMTS12 Protein affects the extracellular matrix, researchers can gain insights into its role in various diseases and disorders, such as osteoarthritis and cancer.

Another potential application of Recombinant Human ADAMTS12 Protein is in drug development. As this protein is involved in extracellular matrix remodeling, it could be a potential target for drugs aimed at treating diseases and disorders related to abnormal extracellular matrix function. Additionally, Recombinant Human ADAMTS12 Protein could also be used in drug screening assays to identify potential inhibitors or activators of its activity.

In the clinical setting, Recombinant Human ADAMTS12 Protein could be used as a diagnostic or prognostic marker for certain diseases and disorders. For example, studies have shown that this protein is upregulated in certain types of cancer, and its levels could potentially be used as a biomarker for early detection or disease progression.

Conclusion

In summary, Recombinant Human ADAMTS12 Protein is a metalloproteinase that plays important roles in extracellular matrix organization, tissue development, and inflammation. Its structure, activity, and potential applications make it a valuable tool for research and drug development. Further studies on this protein