Recombinant Human AMIGO1, N-His

Description of Recombinant Human AMIGO1, N-His

Introduction

Recombinant Human AMIGO1, also known as Alivin-1 or Amphoterin-induced gene and ORF1, is a protein that plays a crucial role in cell signaling and development. This protein is encoded by the AMIGO1 gene and is found in various tissues and organs in the human body. In this article, we will discuss the structure, activity, and application of Recombinant Human AMIGO1.

Structure of Recombinant Human AMIGO1

Recombinant Human AMIGO1 is a 60 kDa protein with a molecular formula of C₃₃H₄₄N₆O₈S₂. It is composed of 538 amino acids and has a predicted isoelectric point of 6.32. The protein contains a single transmembrane domain, a cytoplasmic tail, and an extracellular domain.

The extracellular domain of Recombinant Human AMIGO1 contains three immunoglobulin-like domains, which are responsible for protein-protein interactions. These domains are essential for the function of AMIGO1 in cell adhesion and signaling. The cytoplasmic tail of AMIGO1 contains a PDZ-binding motif, which allows the protein to interact with other proteins and participate in various signaling pathways.

In addition to the full-length protein, Recombinant Human AMIGO1 can also be produced in a truncated form, lacking the transmembrane and cytoplasmic domains. This truncated form is often used in research and as a therapeutic protein.

Activity of Recombinant Human AMIGO1

Recombinant Human AMIGO1 is involved in various cellular processes, including cell adhesion, migration, and differentiation. It is also known to play a role in neuronal development and synaptic plasticity. AMIGO1 has been shown to interact with several other proteins, such as Neogenin, Plexin-B2, and Ephrin-B3, to regulate these processes.

One of the key functions of AMIGO1 is its role in cell adhesion. The extracellular domains of AMIGO1 can interact with other proteins, such as L1CAM and integrins, to mediate cell-cell and cell-matrix adhesion. This is crucial for the proper development and maintenance of tissues and organs.

In addition to its role in cell adhesion, Recombinant Human AMIGO1 is also involved in cell signaling. The protein has been shown to activate the PI3K-Akt signaling pathway, which is important for cell survival and proliferation. AMIGO1 has also been found to regulate the activity of the MAPK/ERK pathway, which is involved in cell differentiation and migration.

Application of Recombinant Human AMIGO1

Due to its diverse functions and interactions with other proteins, Recombinant Human AMIGO1 has a wide range of potential applications. One of the main applications of this protein is in the field of neuroscience. AMIGO1 has been shown to play a role in neuronal development and synaptic plasticity, making it a potential therapeutic target for neurological disorders.

Recombinant Human AMIGO1 has also been studied for its potential role in cancer. It has been found to be overexpressed in various types of cancer, including breast, lung, and colon cancer. Targeting AMIGO1 could potentially inhibit cancer cell proliferation and migration, making it a promising target for cancer therapy.

In addition, Recombinant Human AMIGO1 has also been investigated for its potential use in tissue engineering. Its role in cell adhesion and signaling makes it a promising candidate for promoting cell growth and tissue regeneration.

Conclusion

Recombinant Human AMIGO1 is a 60 kDa protein with a complex structure and diverse functions. It plays a crucial role in cell