Recombinant Human BCAR3 Protein, N-His

Description of Recombinant Human BCAR3 Protein, N-His

Introduction

Recombinant proteins have become an essential tool in various fields of research, including biotechnology, medicine, and drug development. These proteins are produced through genetic engineering techniques, allowing for the production of large quantities of specific proteins with high purity and consistency. One such recombinant protein is the human BCAR3 protein, which has gained significant attention in recent years due to its role in cancer progression and metastasis. In this article, we will explore the structure, activity, and application of recombinant human BCAR3 protein.

Structure of Recombinant Human BCAR3 Protein

The BCAR3 protein, also known as breast cancer anti-estrogen resistance 3, is a member of the BCAR family of proteins. It is encoded by the BCAR3 gene located on chromosome 16 in humans. The recombinant human BCAR3 protein is produced by inserting the BCAR3 gene into a suitable expression vector and then expressing it in a host cell, typically E. coli or yeast.

The recombinant protein consists of 253 amino acids and has a molecular weight of approximately 28 kDa. It contains several functional domains, including a proline-rich region, a SH2 domain, and a BCAR3-specific domain. These domains play a crucial role in the protein’s interaction with other proteins and its activity.

Activity of Recombinant Human BCAR3 Protein

The BCAR3 protein is primarily known for its role in regulating cell adhesion, migration, and invasion. It functions as an adapter protein, connecting various signaling pathways and promoting their activation. It interacts with multiple proteins, including focal adhesion kinase (FAK), Src, and p130Cas, and modulates their activity.

Studies have shown that overexpression of BCAR3 can promote cell proliferation, migration, and invasion, while its inhibition can suppress these processes. Additionally, BCAR3 has been linked to the development and progression of various cancers, including breast, ovarian, and prostate cancers. It has also been implicated in drug resistance, making it a potential therapeutic target for cancer treatment.

Application of Recombinant Human BCAR3 Protein

The recombinant human BCAR3 protein has several potential applications in research and medicine. One of its main uses is in studying the role of BCAR3 in cancer progression and metastasis. Recombinant BCAR3 protein can be used to study its interaction with other proteins and signaling pathways, as well as its role in promoting cancer cell proliferation, migration, and invasion.

Furthermore, recombinant BCAR3 protein can be used to develop drugs that target BCAR3 and inhibit its activity. This could potentially lead to the development of more effective cancer treatments, especially for drug-resistant cancers.

Another potential application of recombinant BCAR3 protein is in diagnostic tests. As BCAR3 has been linked to various cancers, it could serve as a biomarker for early detection and diagnosis of these cancers. Recombinant BCAR3 protein could be used in diagnostic tests to detect its presence in patient samples, providing valuable information for cancer diagnosis and treatment.

Conclusion

In summary, recombinant human BCAR3 protein is a crucial protein in cancer research, with potential applications in drug development and diagnostics. Its structure, activity, and role in cancer progression make it a promising target for further research and development. With the advancement of genetic engineering techniques, the production of recombinant BCAR3 protein has become more accessible, allowing for more in-depth studies and potential therapeutic applications in the future.