Recombinant Human CATSPERB Protein, N-His

Description of Recombinant Human CATSPERB Protein, N-His

Introduction

Recombinant proteins are proteins that are produced in a laboratory through genetic engineering techniques. These proteins have become essential tools in various fields of research, including biotechnology, medicine, and drug development. One such recombinant protein is the Recombinant Human CATSPERB Protein.

Structure of Recombinant Human CATSPERB Protein

CATSPERB (Cation Channel Sperm Associated Protein B) is a protein that is predominantly expressed in the testis and is involved in sperm function. The recombinant form of this protein is produced by cloning the human CATSPERB gene into a suitable expression vector and then expressing it in a host cell, typically E. coli or yeast.

The recombinant CATSPERB protein consists of 596 amino acids and has a predicted molecular weight of 68 kDa. It contains a transmembrane domain and a C-terminal coiled-coil domain, which are critical for its function. The protein also has several potential phosphorylation sites, indicating its involvement in signaling pathways.

Activity of Recombinant Human CATSPERB Protein

The CATSPERB protein is a member of the CATSPER family, which consists of four proteins (CATSPER1-4) that form a cation channel in the sperm cell membrane. This channel is essential for the influx of calcium ions, which is crucial for sperm motility and fertilization.

The recombinant CATSPERB protein has been shown to interact with other CATSPER proteins and form functional channels in vitro. It has also been demonstrated to enhance the sperm’s ability to penetrate the egg during fertilization. This suggests that the recombinant CATSPERB protein can be used as a tool to study the role of CATSPER channels in sperm function and fertilization.

Application of Recombinant Human CATSPERB Protein

The recombinant CATSPERB protein has various applications in both research and clinical settings. One of the main applications is in the study of male infertility. Mutations in the CATSPER genes have been linked to male infertility, and the recombinant protein can be used to study the functional consequences of these mutations.

Furthermore, the recombinant CATSPERB protein can also be used in drug development for male contraception. As the CATSPER channels are essential for sperm function, targeting them with specific inhibitors can potentially lead to the development of non-hormonal male contraceptives.

In addition, the recombinant CATSPERB protein has potential diagnostic applications. Studies have shown that the levels of CATSPER proteins are altered in infertile men, making them potential biomarkers for male infertility.

Conclusion

The recombinant Human CATSPERB protein is a valuable tool in the study of sperm function and male infertility. Its structure, activity, and applications make it a versatile protein with potential uses in various fields of research and medicine. Further studies on this protein can provide valuable insights into male fertility and lead to the development of novel therapies for male infertility.