Recombinant Human CAVIN3 Protein, N-His-SUMO & C-Strep

Description of Recombinant Human CAVIN3 Protein, N-His-SUMO & C-Strep

Introduction to Recombinant Human CAVIN3 Protein

Recombinant Human CAVIN3 protein, also known as Caveolae-associated protein 3, is a member of the Caveolae-associated protein (CAVIN) family. This protein plays a crucial role in the formation and maintenance of caveolae, small invaginations of the plasma membrane that are involved in various cellular processes such as endocytosis, lipid metabolism, and signal transduction. Recombinant Human CAVIN3 protein is produced through recombinant DNA technology, making it a valuable tool for studying the structure, function, and applications of this protein.

Structure of Recombinant Human CAVIN3 Protein

Recombinant Human CAVIN3 protein is a 25 kDa protein composed of 226 amino acids. It contains a conserved N-terminal domain, a central coiled-coil domain, and a C-terminal domain. The N-terminal domain is responsible for the interaction with other CAVIN proteins, while the coiled-coil domain is involved in the formation of the caveolar coat. The C-terminal domain contains a highly conserved region that is essential for the localization of CAVIN3 to caveolae. Recombinant Human CAVIN3 protein also contains multiple phosphorylation sites, which play a role in regulating its activity.

Activity of Recombinant Human CAVIN3 Protein

The main function of CAVIN3 protein is to regulate the formation and stability of caveolae. It does so by interacting with other CAVIN proteins, such as CAVIN1 and CAVIN2, to form a complex that binds to caveolin, the structural protein of caveolae. This complex is essential for the proper localization of caveolin to the plasma membrane and the formation of caveolae. Recombinant Human CAVIN3 protein has also been shown to play a role in the regulation of lipid metabolism and insulin signaling.

Moreover, recent studies have revealed that CAVIN3 protein is involved in the regulation of cellular signaling pathways. It has been shown to interact with various signaling proteins, such as epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor (PDGFR), and modulate their activity. This suggests that Recombinant Human CAVIN3 protein may have a broader role in cellular processes beyond caveolae formation.

Applications of Recombinant Human CAVIN3 Protein

Recombinant Human CAVIN3 protein has various applications in both research and therapeutic settings. Its role in the formation and maintenance of caveolae makes it a valuable tool for studying the structure and function of these structures. Researchers can use Recombinant Human CAVIN3 protein to investigate the role of caveolae in different cellular processes and to understand the mechanisms of caveolae-related diseases.

Furthermore, the involvement of CAVIN3 protein in cellular signaling pathways makes it a potential target for therapeutic interventions. Dysregulation of caveolae and their associated proteins has been linked to various diseases, such as cancer, cardiovascular diseases, and metabolic disorders. Recombinant Human CAVIN3 protein can be used to develop drugs that target this protein and modulate its activity, potentially providing a new avenue for treating these diseases.

Conclusion

In summary, Recombinant Human CAVIN3 protein is a crucial player in the formation and maintenance of caveolae, with potential roles in lipid metabolism, signaling pathways, and disease development. Its production through recombinant DNA technology provides a valuable tool for studying the structure, function, and applications of this protein. Further research on Recombinant Human CAVIN3 protein may lead to a better understanding of its role in cellular processes and its potential as a therapeutic target.