Recombinant Human CCT3 Protein, N-His

Description of Recombinant Human CCT3 Protein, N-His

Introduction

Recombinant Human CCT3 Protein, also known as chaperonin-containing TCP-1 subunit 3, is a protein that plays a crucial role in protein folding and assembly. It is a member of the chaperonin family of proteins, which are responsible for assisting in the proper folding of newly synthesized proteins in the cell. In this article, we will discuss the structure, activity, and application of Recombinant Human CCT3 Protein.

Structure of Recombinant Human CCT3 Protein

Recombinant Human CCT3 Protein is a 574 amino acid long protein with a molecular weight of approximately 60 kDa. It is composed of two domains, an N-terminal domain and a C-terminal domain, connected by a flexible linker region. The N-terminal domain contains a nucleotide-binding domain, which is responsible for binding to ATP and regulating the activity of the protein. The C-terminal domain is involved in substrate binding and protein folding.

Activity of Recombinant Human CCT3 Protein

The main activity of Recombinant Human CCT3 Protein is to act as a molecular chaperone, assisting in the proper folding of newly synthesized proteins. It is a part of the chaperonin complex, which is composed of eight subunits, including CCT3. This complex is responsible for folding and assembling proteins in the cell. Recombinant Human CCT3 Protein specifically assists in the folding of cytoskeletal proteins, such as actin and tubulin, and also plays a role in the folding of other essential proteins, including heat shock proteins and enzymes.

Application of Recombinant Human CCT3 Protein

Recombinant Human CCT3 Protein has various applications in the field of biochemistry and molecular biology. Some of the notable applications are discussed below.

Production of Recombinant Proteins

Recombinant Human CCT3 Protein can be used in the production of recombinant proteins in various expression systems. It can be co-expressed with the target protein to assist in its proper folding and assembly. This ensures the production of functional and correctly folded recombinant proteins, which is essential for their use in various applications.

Antigen Presentation

Recombinant Human CCT3 Protein has also been shown to play a role in antigen presentation. It is involved in the folding and assembly of major histocompatibility complex (MHC) class I molecules, which are responsible for presenting antigens to immune cells. This suggests that Recombinant Human CCT3 Protein may have potential applications in vaccine development and immunotherapy.

Drug Target

As Recombinant Human CCT3 Protein is involved in the folding and assembly of essential proteins, it has been identified as a potential drug target. Inhibition of its activity can lead to misfolding of critical proteins and disruption of cellular processes, making it a potential target for the treatment of various diseases, including cancer and neurodegenerative disorders.

Diagnostic Marker

Studies have shown that the expression of Recombinant Human CCT3 Protein is altered in various diseases, including cancer and viral infections. This suggests that it can serve as a diagnostic marker for these diseases. Detection of changes in its expression levels can aid in early diagnosis and monitoring of disease progression.

Research Tool

Recombinant Human CCT3 Protein can also be used as a research tool for studying protein folding and assembly. Its activity can be manipulated to study the effects of chaperonin deficiency on cellular processes. It can also be used to investigate the role of chaperonins in diseases and to screen potential inhibitors for therapeutic purposes.

Conclusion

In conclusion, Recombinant Human CCT3 Protein is a crucial protein involved in protein folding and assembly. Its structure, activity, and various applications make it a valuable tool in the fields of biochemistry and molecular biology. Further research on this protein may lead to the development of new therapies and diagnostic tools for various diseases.