Recombinant Human CISD1 Protein, N-His-SUMO

Description of Recombinant Human CISD1 Protein, N-His-SUMO

Title: Introduction to Recombinant Human CISD1 Protein

Recombinant Human CISD1 Protein, also known as CDGSH iron sulfur domain-containing protein 1, is a highly conserved protein that plays a crucial role in maintaining cellular homeostasis. This protein is encoded by the CISD1 gene and is found in the mitochondria of eukaryotic cells. In recent years, recombinant human CISD1 protein has gained significant attention due to its potential therapeutic applications in various diseases. In this article, we will provide a comprehensive overview of the structure, activity, and applications of recombinant human CISD1 protein.

Title: Structure of Recombinant Human CISD1 Protein

The recombinant human CISD1 protein is a small, 14 kDa protein that contains a conserved CDGSH iron-sulfur domain at its N-terminus. This domain is essential for the protein’s function, as it binds to iron-sulfur clusters and facilitates their transfer to other proteins. The C-terminus of the protein contains a transmembrane domain, which anchors the protein to the inner mitochondrial membrane. This unique structure allows the protein to interact with other mitochondrial proteins and play a vital role in maintaining mitochondrial function.

Title: Activity of Recombinant Human CISD1 Protein

The primary function of recombinant human CISD1 protein is to regulate iron-sulfur cluster biogenesis and transfer in the mitochondria. Iron-sulfur clusters are essential for various cellular processes, including respiration, DNA repair, and metabolism. CISD1 protein interacts with other proteins, such as frataxin and ISCU, to facilitate the formation and transfer of iron-sulfur clusters. This activity is crucial for maintaining the proper functioning of mitochondria and preventing oxidative stress.

Moreover, recombinant human CISD1 protein has been shown to play a role in regulating apoptosis, or programmed cell death. Studies have demonstrated that overexpression of CISD1 protein can protect cells from apoptosis induced by oxidative stress, while its depletion can enhance apoptosis. This suggests that recombinant human CISD1 protein may have therapeutic potential in diseases characterized by excessive cell death, such as neurodegenerative disorders.

Title: Applications of Recombinant Human CISD1 Protein

The unique structure and activity of recombinant human CISD1 protein make it a promising candidate for various therapeutic applications. One potential application is in the treatment of neurodegenerative disorders, such as Parkinson’s and Alzheimer’s disease. These diseases are characterized by mitochondrial dysfunction and oxidative stress, which can be mitigated by the activity of CISD1 protein. Studies have shown that overexpression of CISD1 protein can protect neurons from oxidative stress and improve mitochondrial function, making it a potential therapeutic target for these diseases.

Another potential application of recombinant human CISD1 protein is in cancer treatment. CISD1 protein has been found to be downregulated in various types of cancer, and its overexpression has been shown to inhibit tumor growth and promote cell death. This suggests that recombinant human CISD1 protein may have anti-cancer properties and could be used as a targeted therapy for certain types of cancer.

In addition to its therapeutic applications, recombinant human CISD1 protein also has diagnostic potential. CISD1 protein has been identified as a potential biomarker for various diseases, including cardiovascular diseases and diabetes. Its levels have been found to be altered in patients with these conditions, making it a potential diagnostic marker for early detection and monitoring of disease progression.

Title: Conclusion

In summary, recombinant human CISD1 protein is a small but crucial protein that plays a vital role in maintaining cellular homeostasis. Its unique structure and activity make it a promising candidate for various therapeutic and diagnostic applications. Further research and development of recombinant human CISD1 protein could lead to the development of new treatments for diseases such as neurodegenerative disorders and cancer, ultimately improving the quality of life for patients.