Recombinant Human DSPP, N-His

Description of Recombinant Human DSPP, N-His

Introduction

Recombinant Human DSPP (Dentin Sialophosphoprotein) is a protein that plays a crucial role in the formation and mineralization of teeth. It is a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family, which is known for its involvement in the regulation of bone and tooth development. Recombinant Human DSPP is produced through recombinant DNA technology and has a wide range of applications in dental research and clinical practice.

Structure of Recombinant Human DSPP

Recombinant Human DSPP is a large protein consisting of 1,330 amino acids. It is mainly composed of two major domains: the dentin sialoprotein (DSP) domain and the dentin phosphoprotein (DPP) domain. The DSP domain is located at the N-terminus and contains 10 repeats of the highly conserved DSP motif. This domain is responsible for the binding of DSPP to collagen, which is essential for the formation of mineralized tissues. The DPP domain, on the other hand, is located at the C-terminus and contains multiple phosphorylated serine residues. This domain is involved in the regulation of mineralization and has been shown to interact with various proteins and enzymes involved in tooth development.

Activity of Recombinant Human DSPP

Recombinant Human DSPP has been extensively studied for its role in tooth development and mineralization. It has been shown to play a crucial role in the formation of dentin, the hard tissue that makes up the majority of the tooth structure. DSPP is secreted by odontoblasts, the cells responsible for dentin formation, and is cleaved into two smaller proteins, DSP and DPP. These proteins then interact with other molecules in the extracellular matrix to promote the mineralization process.

In addition to its role in tooth development, Recombinant Human DSPP has also been found to have antimicrobial properties. It has been shown to inhibit the growth of bacteria commonly found in the oral cavity, such as Streptococcus mutans, which is known to cause tooth decay. This antimicrobial activity of DSPP makes it a potential candidate for the development of new dental treatments and products.

Applications of Recombinant Human DSPP

Recombinant Human DSPP has a wide range of applications in dental research and clinical practice. One of its main uses is in the study of tooth development and mineralization. Researchers use recombinant DSPP to better understand the mechanisms involved in these processes and to develop new treatments for dental diseases such as tooth decay and dentin hypersensitivity.

Another important application of Recombinant Human DSPP is in the development of dental biomaterials. DSPP has been shown to enhance the adhesion and proliferation of dental stem cells, making it a promising candidate for the development of new biomaterials for tooth repair and regeneration. Its antimicrobial properties also make it a potential ingredient in dental materials to prevent bacterial growth and promote healing.

In addition, Recombinant Human DSPP is also used in diagnostic tests for dental diseases. Its presence in the saliva has been linked to various dental conditions, and its detection can aid in the early diagnosis and treatment of these diseases.

Conclusion

Recombinant Human DSPP is a crucial protein in tooth development and mineralization. Its structure, activity, and wide range of applications make it a valuable tool in dental research and clinical practice. With further studies and advancements in technology, this protein holds great potential for the development of new dental treatments and products.