Recombinant Human EFNA3 Protein, N-His-SUMO

Description of Recombinant Human EFNA3 Protein, N-His-SUMO

Introduction

Recombinant Human EFNA3 Protein, also known as ephrin-A3, is a member of the ephrin family of proteins. It is a glycosylphosphatidylinositol (GPI)-anchored protein that plays a crucial role in cell signaling and tissue development. In this article, we will discuss the structure, activity, and application of Recombinant Human EFNA3 Protein.

Structure of Recombinant Human EFNA3 Protein

Recombinant Human EFNA3 Protein is a 33 kDa protein consisting of 231 amino acids. It contains a conserved N-terminal ephrin domain, a transmembrane region, and a C-terminal GPI anchor. The ephrin domain is responsible for binding to its receptor, EphA receptors, and initiating downstream signaling pathways.

Activity of Recombinant Human EFNA3 Protein

Recombinant Human EFNA3 Protein is a potent ligand for EphA receptors, specifically EphA4 and EphA5. Binding of EFNA3 to these receptors leads to the activation of multiple signaling pathways, including the mitogen-activated protein kinase (MAPK) pathway and the phosphoinositide 3-kinase (PI3K) pathway. These pathways are involved in cell proliferation, survival, and differentiation.

In addition to its role in cell signaling, EFNA3 also plays a crucial role in tissue development. It is involved in axon guidance, neuronal migration, and synapse formation. EFNA3 is also known to regulate cell adhesion and motility, which are important processes in tissue development and wound healing.

Application of Recombinant Human EFNA3 Protein

Due to its role in cell signaling and tissue development, Recombinant Human EFNA3 Protein has various applications in the field of research and medicine. Some of these applications include:

1. Recombinant Protein Production

Recombinant Human EFNA3 Protein is widely used as a recombinant protein in various research studies. It can be produced in large quantities using recombinant DNA technology, making it easily accessible for research purposes.

2. Antigen for Antibody Production

EFNA3 has been identified as a potential antigen for antibody production. Antibodies against EFNA3 can be used to study its expression and function in different tissues and cell types. These antibodies can also be used in diagnostic tests for diseases associated with EFNA3 dysregulation.

3. Potential Therapeutic Target

The dysregulation of EFNA3 has been linked to various diseases, including cancer, Alzheimer’s disease, and multiple sclerosis. Therefore, EFNA3 is being explored as a potential therapeutic target for these diseases. Recombinant Human EFNA3 Protein can be used in preclinical studies to evaluate its efficacy as a therapeutic target.

4. Tissue Engineering

EFNA3’s role in tissue development and wound healing makes it a potential candidate for tissue engineering applications. Recombinant Human EFNA3 Protein can be used to promote cell adhesion and migration, leading to the formation of functional tissues.

5. Drug Screening

The dysregulation of EFNA3 has been implicated in drug resistance in cancer cells. Recombinant Human EFNA3 Protein can be used in drug screening assays to identify potential compounds that can target EFNA3 and overcome drug resistance.

Conclusion

In summary, Recombinant Human EFNA3 Protein is a crucial protein involved in cell signaling and tissue development. Its structure, activity, and various applications make it a valuable tool in research and medicine. Further studies on EFNA3 and its interactions with other proteins and receptors may lead to the development of new therapies for diseases associated with its dysregulation.