Recombinant Human HINT1 Protein, N-His

Description of Recombinant Human HINT1 Protein, N-His

Introduction

Recombinant Human HINT1 Protein, also known as Histidine Triad Nucleotide-Binding Protein 1, is a highly conserved enzyme that plays a crucial role in various biological processes. This protein is encoded by the HINT1 gene and is found in a wide range of organisms, including humans. Recombinant Human HINT1 Protein has been extensively studied for its structure, activity, and potential applications in various fields.

Structure of Recombinant Human HINT1 Protein

Recombinant Human HINT1 Protein is a small enzyme with a molecular weight of approximately 14 kDa. It is composed of 126 amino acids and is conserved across species, with a 97% similarity between human and mouse sequences. The protein has a characteristic histidine triad motif, which is essential for its enzymatic activity. It also contains a nucleotide-binding site and a hydrophobic pocket that is responsible for its interactions with other molecules.

Activity of Recombinant Human HINT1 Protein

Recombinant Human HINT1 Protein is a hydrolase enzyme that catalyzes the hydrolysis of adenosine 5′-monophosphate (AMP) and other nucleotides. It has a high specificity for AMP and can also hydrolyze other nucleotides, such as ADP and ATP, albeit with lower efficiency. This enzyme plays a critical role in maintaining cellular homeostasis by regulating the levels of nucleotides, which are essential for various cellular processes. It also has a role in DNA repair and apoptosis, making it a crucial enzyme in maintaining the integrity of the cell.

Applications of Recombinant Human HINT1 Protein

Recombinant Human HINT1 Protein has several potential applications in the fields of medicine, biotechnology, and biochemistry.

Antigen for Antibody Production

Recombinant Human HINT1 Protein can be used as an antigen to produce specific antibodies for research and diagnostic purposes. Antibodies against this protein can be used to study its expression and localization in different tissues and cell types. They can also be used in immunohistochemistry and western blotting techniques to detect changes in HINT1 levels under different conditions.

Drug Target

Recombinant Human HINT1 Protein has been identified as a potential drug target for various diseases, including cancer and neurodegenerative disorders. Its role in regulating nucleotide levels and DNA repair makes it an attractive target for developing drugs that can modulate its activity. Inhibitors of HINT1 have been shown to have anticancer activity, making this protein a promising target for cancer therapy.

Biomarker for Disease Diagnosis

The expression levels of Recombinant Human HINT1 Protein have been found to be altered in several diseases, including Alzheimer’s, Huntington’s, and Parkinson’s disease. This makes it a potential biomarker for early diagnosis and monitoring disease progression. Its levels can be measured in biological fluids, such as blood and cerebrospinal fluid, to assess its potential as a diagnostic marker.

Role in Neuroprotection

Recombinant Human HINT1 Protein has been shown to have a neuroprotective role in several neurodegenerative disorders. Its ability to regulate nucleotide levels and promote DNA repair makes it a potential therapeutic target for these diseases. Studies have shown that HINT1 can protect against oxidative stress and reduce neuronal damage, making it a promising candidate for the treatment of neurodegenerative diseases.

Industrial Applications

Recombinant Human HINT1 Protein has also been studied for its potential industrial applications. Its ability to hydrolyze nucleotides has been utilized in the production of nucleotide analogs, which are used as drugs and research tools. It has also been investigated for its role in improving the efficiency of biofuel production by reducing the levels of inhibitory nucleotides in the fermentation process.

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