Recombinant Human MTHFD1 Protein, N-His

Description of Recombinant Human MTHFD1 Protein, N-His

Introduction to Recombinant Human MTHFD1 Protein

Recombinant Human MTHFD1 Protein, also known as methylenetetrahydrofolate dehydrogenase 1, is a key enzyme involved in the folate metabolism pathway. This protein is encoded by the MTHFD1 gene and is responsible for converting 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate, an essential step in the synthesis of purines and thymidylate, which are important for DNA and RNA synthesis. Recombinant Human MTHFD1 Protein is produced through genetic engineering techniques, making it a valuable tool for research and therapeutic applications.

Structure of Recombinant Human MTHFD1 Protein

Recombinant Human MTHFD1 Protein is a homodimer, meaning it is composed of two identical subunits. Each subunit has a molecular weight of approximately 105 kDa and is composed of 913 amino acids. The structure of this protein has been extensively studied and it has been found to have a conserved NAD(P) binding domain, a catalytic domain, and a regulatory domain. The catalytic domain is responsible for the conversion of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate, while the regulatory domain controls the activity of the enzyme.

Activity of Recombinant Human MTHFD1 Protein

The main function of Recombinant Human MTHFD1 Protein is to catalyze the reversible conversion of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate. This reaction is essential for the synthesis of purines and thymidylate, which are building blocks for DNA and RNA. Additionally, this protein is also involved in the production of S-adenosylmethionine, a key molecule in various cellular processes such as methylation, which is important for gene regulation and protein function.

Research has shown that Recombinant Human MTHFD1 Protein has a higher affinity for NADP+ than NAD+. This preference for NADP+ makes this protein an important regulator of cellular redox balance. It has been suggested that this enzyme may play a role in protecting cells from oxidative stress by maintaining a reduced state of NADP+.

Applications of Recombinant Human MTHFD1 Protein

The production of Recombinant Human MTHFD1 Protein has opened up new opportunities for research and therapeutic applications. This protein can be used as a tool for studying the folate metabolism pathway and its role in various cellular processes. It can also be used to investigate the role of MTHFD1 gene mutations in diseases such as cancer and neurological disorders.

Recombinant Human MTHFD1 Protein has also been used in the development of diagnostic tests for folate deficiency. Folate deficiency is a common condition that can lead to various health problems, and the ability to accurately measure MTHFD1 activity can aid in the diagnosis and treatment of this condition.

Furthermore, Recombinant Human MTHFD1 Protein has shown potential as a therapeutic target for cancer treatment. Studies have shown that inhibiting the activity of this enzyme can lead to decreased cancer cell proliferation and increased sensitivity to chemotherapy drugs. This makes Recombinant Human MTHFD1 Protein a promising target for the development of new cancer treatments.

Conclusion

Recombinant Human MTHFD1 Protein is a key enzyme involved in the folate metabolism pathway. Its structure, activity, and applications have been extensively studied and it has been found to play important roles in various cellular processes. The production of this protein through genetic engineering techniques has opened up new opportunities for research and therapeutic applications, making it a valuable tool in the