Recombinant Human NECTIN4/PVRL4 Protein, N-His

Description of Recombinant Human NECTIN4/PVRL4 Protein, N-His

Introduction to Recombinant Human NECTIN4/PVRL4 Protein

Recombinant Human NECTIN4/PVRL4 Protein, also known as Poliovirus Receptor-Related 4 (PVRL4), is a cell adhesion molecule that plays a crucial role in various cellular processes such as cell-cell adhesion, cell migration, and signal transduction. This protein is encoded by the PVRL4 gene and is a member of the NECTIN family of proteins, which are transmembrane glycoproteins involved in cell-cell interactions.

Structure of Recombinant Human NECTIN4/PVRL4 Protein

The recombinant human NECTIN4/PVRL4 protein is a 55-kDa protein composed of 477 amino acids. It consists of a signal peptide, an extracellular domain, a transmembrane domain, and a cytoplasmic domain. The extracellular domain contains three immunoglobulin-like domains (Ig-like domains) that are responsible for binding to its ligands. The transmembrane domain anchors the protein to the cell membrane, while the cytoplasmic domain interacts with various intracellular proteins to mediate signal transduction.

Activity of Recombinant Human NECTIN4/PVRL4 Protein

Recombinant Human NECTIN4/PVRL4 Protein is primarily involved in cell-cell adhesion, which is essential for maintaining tissue integrity and regulating cell migration. It mediates cell-cell adhesion by interacting with its ligands, including other NECTIN family members, such as NECTIN1 and NECTIN3, and the herpes simplex virus glycoprotein D. This interaction leads to the formation of adherens junctions and tight junctions, which are crucial for cell adhesion and communication between cells.

Besides its role in cell adhesion, Recombinant Human NECTIN4/PVRL4 Protein also plays a role in signal transduction. The cytoplasmic domain of this protein interacts with various intracellular proteins, such as afadin and the Rho GTPase family, to regulate cell migration and cytoskeletal organization. It has also been shown to activate the AKT signaling pathway, which is involved in cell survival and proliferation, and the ERK1/2 signaling pathway, which is essential for cell growth and differentiation.

Application of Recombinant Human NECTIN4/PVRL4 Protein

Recombinant Human NECTIN4/PVRL4 Protein has various applications in both research and clinical settings. In research, this protein is used as a tool to study cell adhesion, cell migration, and signal transduction. It is also used to investigate the interaction between NECTIN family members and their ligands, as well as the role of these interactions in various cellular processes.

In clinical settings, Recombinant Human NECTIN4/PVRL4 Protein has been studied for its potential as a therapeutic target in cancer treatment. NECTIN4 is overexpressed in various types of cancer, including breast, lung, and ovarian cancer, and has been associated with tumor growth, invasion, and metastasis. Therefore, targeting NECTIN4 with antibodies or small molecule inhibitors may provide a promising approach for cancer therapy.

Moreover, Recombinant Human NECTIN4/PVRL4 Protein has also been investigated as a potential biomarker for cancer diagnosis and prognosis. Studies have shown that NECTIN4 expression levels in tumor tissues may serve as a prognostic indicator for cancer patients, and NECTIN4-specific antibodies can be used for targeted imaging and detection of cancer cells.

Conclusion

In summary, Recombinant Human NECTIN4/PVRL4 Protein is a key player in cell adhesion and signal transduction, with various applications in research and clinical settings. Its structural features, activity, and potential applications make it a