Recombinant Human NEIL3 Protein, N-His

Description of Recombinant Human NEIL3 Protein, N-His

Introduction

Recombinant Human NEIL3 Protein is a type of recombinant protein that has been extensively studied for its structure, activity, and potential applications in various fields of research. This protein belongs to the NEIL family of DNA glycosylases, which are enzymes involved in DNA repair mechanisms. In this article, we will explore the structure, activity, and potential applications of Recombinant Human NEIL3 Protein.

Structure of Recombinant Human NEIL3 Protein

Recombinant Human NEIL3 Protein is a 429 amino acid long protein with a molecular weight of approximately 48 kDa. It contains a highly conserved catalytic domain, which is responsible for its DNA glycosylase activity. This domain is composed of a helix-two-turn-helix motif and a zinc-binding motif, which are essential for the catalytic activity of the protein.

The protein also contains a C-terminal domain, which is involved in protein-protein interactions and is essential for its function in DNA repair. The C-terminal domain is also responsible for the nuclear localization of the protein, as Recombinant Human NEIL3 Protein is predominantly found in the nucleus of cells.

Activity of Recombinant Human NEIL3 Protein

Recombinant Human NEIL3 Protein is a DNA glycosylase, which means it has the ability to recognize and remove damaged bases from DNA. Specifically, it is involved in the repair of oxidative DNA damage, which is caused by reactive oxygen species (ROS). This type of damage can lead to mutations and ultimately, diseases such as cancer.

The activity of Recombinant Human NEIL3 Protein is dependent on its catalytic domain, which is responsible for binding to damaged DNA and cleaving the glycosidic bond between the damaged base and the sugar backbone of DNA. This results in the removal of the damaged base, which is then replaced with a correct base by other DNA repair enzymes.

Applications of Recombinant Human NEIL3 Protein

Recombinant Human NEIL3 Protein has potential applications in various fields of research, including cancer biology, aging, and neurodegenerative diseases. Its role in repairing oxidative DNA damage makes it a potential target for cancer therapy. In fact, studies have shown that NEIL3 expression is dysregulated in various types of cancer, and targeting this protein could potentially lead to the development of new cancer treatments.

In addition, Recombinant Human NEIL3 Protein has also been linked to aging and age-related diseases. As we age, our cells accumulate DNA damage, which can lead to cellular dysfunction and ultimately, age-related diseases. By understanding the role of NEIL3 in DNA repair, researchers hope to develop interventions that can delay or prevent age-related diseases.

Furthermore, Recombinant Human NEIL3 Protein has also been implicated in neurodegenerative diseases such as Alzheimer’s and Parkinson’s. These diseases are characterized by the accumulation of damaged DNA in neurons, leading to cellular dysfunction and ultimately, neuronal death. By studying the role of NEIL3 in DNA repair, researchers hope to develop treatments that can slow down or reverse the progression of these diseases.

Conclusion

In summary, Recombinant Human NEIL3 Protein is a DNA glycosylase that plays a crucial role in repairing oxidative DNA damage. Its structure, activity, and potential applications make it a highly studied protein in the field of DNA repair and various other areas of research. Further studies on this protein could potentially lead to the development of new treatments for cancer, aging, and neurodegenerative diseases.