Recombinant Human P4HA1 Protein, N-His

Description of Recombinant Human P4HA1 Protein, N-His

Introduction

Recombinant Human P4HA1 Protein, also known as Prolyl 4-hydroxylase subunit alpha-1, is a type of recombinant protein that plays a crucial role in the post-translational modification of collagen. This protein is encoded by the P4HA1 gene and is involved in the hydroxylation of proline residues in collagen, which is essential for the stability and function of this important structural protein. In this article, we will explore the structure, activity, and application of Recombinant Human P4HA1 Protein.

Structure of Recombinant Human P4HA1 Protein

Recombinant Human P4HA1 Protein is a homodimer, meaning it is composed of two identical subunits. Each subunit consists of three domains: the N-terminal domain, the catalytic domain, and the C-terminal domain. The N-terminal domain is responsible for binding to the collagen substrate, while the catalytic domain contains the active site where the hydroxylation of proline residues takes place. The C-terminal domain is involved in the interaction with other subunits to form the functional homodimer.

The structure of Recombinant Human P4HA1 Protein is highly conserved among different species, indicating its importance in collagen modification. The crystal structure of this protein has been determined, providing valuable insights into its function and potential for drug design.

Activity of Recombinant Human P4HA1 Protein

The main function of Recombinant Human P4HA1 Protein is to catalyze the hydroxylation of proline residues in collagen. This process is essential for the proper folding, stability, and function of collagen. Without proline hydroxylation, collagen would not be able to form its characteristic triple helix structure, which is crucial for its role as a structural protein in tissues such as bone, cartilage, and skin.

In addition to its role in collagen modification, Recombinant Human P4HA1 Protein has been found to be involved in other cellular processes. It has been shown to play a role in the regulation of cell proliferation, differentiation, and migration. This suggests that this protein may have potential applications in tissue engineering and regenerative medicine.

Application of Recombinant Human P4HA1 Protein

Recombinant Human P4HA1 Protein has a wide range of potential applications, both in basic research and in the development of therapeutics. One of the main applications of this protein is in the production of recombinant collagen for use in tissue engineering and regenerative medicine. By hydroxylating proline residues in recombinant collagen, Recombinant Human P4HA1 Protein can improve the stability and functionality of the collagen, making it a more suitable biomaterial for various tissue engineering applications.

In addition, Recombinant Human P4HA1 Protein has also been studied as a potential therapeutic target for diseases related to collagen dysfunction. For example, mutations in the P4HA1 gene have been linked to certain types of osteogenesis imperfecta, a genetic disorder characterized by brittle bones. By targeting Recombinant Human P4HA1 Protein, it may be possible to develop treatments for this and other collagen-related diseases.

Furthermore, Recombinant Human P4HA1 Protein has also been used in research studies to investigate the role of collagen in various diseases, such as cancer and fibrosis. By understanding the function and activity of this protein, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially develop new treatments.

Conclusion

In summary, Recombinant Human P4HA1 Protein is a crucial enzyme involved in the post-translational modification of collagen. Its structure, activity, and potential applications make it a valuable tool for both basic research and the development of therapeutics. As our understanding of the role of collagen in various diseases continues to grow, the importance of Recombinant Human P4HA1 Protein in these areas will only become more apparent.