Recombinant Human PIKFYVE Protein, N-His

Description of Recombinant Human PIKFYVE Protein, N-His

Introduction

Recombinant Human PIKFYVE Protein, also known as Phosphoinositide kinase, FYVE-type zinc finger containing, is a protein that plays a crucial role in regulating the intracellular levels of phosphatidylinositol 3-phosphate (PI3P). This protein is encoded by the PIKFYVE gene and has been extensively studied for its structure, activity, and various applications in the field of cell biology and disease research.

Structure of Recombinant Human PIKFYVE Protein

Recombinant Human PIKFYVE Protein is a 223 kDa protein that consists of 2306 amino acids. It contains a FYVE domain, which is responsible for binding to PI3P, and a kinase domain, which is responsible for phosphorylating PI3P. In addition, this protein also contains a zinc finger domain and a proline-rich domain, which are involved in protein-protein interactions.

The crystal structure of Recombinant Human PIKFYVE Protein has been determined, revealing a unique arrangement of the FYVE and kinase domains. The FYVE domain forms a curved structure that wraps around the kinase domain, creating a pocket for PI3P binding. This structural feature is essential for the specific recognition and binding of PI3P by the protein.

Activity of Recombinant Human PIKFYVE Protein

Recombinant Human PIKFYVE Protein is a lipid kinase that phosphorylates PI3P to produce phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2). This activity is crucial for maintaining the proper levels of PI3P in the cell, which is involved in various cellular processes such as endocytosis, autophagy, and lysosomal function. In addition, Recombinant Human PIKFYVE Protein also plays a role in regulating the levels of other phosphoinositides, such as PI(3,4)P2 and PI(3,4,5)P3, which are involved in signaling pathways.

The activity of Recombinant Human PIKFYVE Protein is tightly regulated by various factors, including its binding to PI3P, its interaction with other proteins, and its localization to specific cellular compartments. Dysregulation of this protein has been linked to various diseases, including neurodegenerative disorders, cancer, and metabolic diseases.

Applications of Recombinant Human PIKFYVE Protein

Recombinant Human PIKFYVE Protein has been widely used in various research applications, including studying its role in cellular processes and its involvement in disease pathogenesis. Its ability to specifically bind to PI3P and regulate its levels makes it a valuable tool for studying the function of PI3P in different cellular contexts.

In addition, Recombinant Human PIKFYVE Protein has also been used in drug discovery and development. Its involvement in various diseases has made it a potential therapeutic target, and inhibitors of this protein have been developed for the treatment of diseases such as Parkinson’s disease and cancer.

Furthermore, Recombinant Human PIKFYVE Protein has also been used in diagnostic assays for various diseases. Its ability to specifically bind to PI3P has been utilized in developing assays for the detection of PI3P levels in patient samples, which can serve as a biomarker for certain diseases.

Conclusion

Recombinant Human PIKFYVE Protein is a crucial protein involved in regulating the levels of PI3P in the cell. Its unique structure and activity make it a valuable tool for studying cellular processes and its involvement in disease pathogenesis. Its potential as a therapeutic target and diagnostic biomarker further highlights the importance of this protein in the field of cell biology and disease research.