Recombinant Human PLAGL2 Protein, N-His

Description of Recombinant Human PLAGL2 Protein, N-His

Introduction

Recombinant Human PLAGL2 Protein, also known as pleiomorphic adenoma gene-like 2 protein, is a key transcription factor that plays a crucial role in various cellular processes such as cell proliferation, differentiation, and apoptosis. This protein is encoded by the PLAGL2 gene and is highly conserved among different species, including humans.

Structure of Recombinant Human PLAGL2 Protein

The recombinant human PLAGL2 protein is a 54 kDa protein consisting of 490 amino acids. It contains a highly conserved zinc finger domain at the C-terminus, which is essential for its DNA binding and transcriptional activation functions. This protein also has a proline-rich region at the N-terminus, which is involved in protein-protein interactions.

Activity of Recombinant Human PLAGL2 Protein

Recombinant human PLAGL2 protein is a transcription factor that regulates gene expression by binding to specific DNA sequences in the promoter regions of target genes. It can act as both a transcriptional activator and repressor, depending on the target gene and the cellular context. PLAGL2 has been shown to interact with various transcriptional co-regulators, such as p300 and CBP, to modulate gene expression.

One of the key functions of PLAGL2 is its role in cell proliferation. Studies have shown that this protein promotes cell cycle progression by activating the expression of cell cycle regulators, such as cyclin D1 and cyclin-dependent kinase 4 (CDK4). PLAGL2 has also been implicated in cell differentiation processes, where it can act as a transcriptional repressor to inhibit the expression of differentiation-associated genes.

Moreover, PLAGL2 has been shown to play a crucial role in apoptosis, or programmed cell death. It has been reported to induce apoptosis in various cancer cell lines by activating the expression of pro-apoptotic genes and inhibiting the expression of anti-apoptotic genes. This suggests that PLAGL2 may have a potential role in cancer therapy.

Application of Recombinant Human PLAGL2 Protein

Recombinant human PLAGL2 protein has a wide range of applications in both basic research and clinical settings. Its role in cell proliferation and differentiation makes it a valuable tool for studying these processes in various cell types. PLAGL2 can also be used to investigate the mechanisms of gene regulation and transcriptional control.

In cancer research, PLAGL2 has emerged as a potential therapeutic target due to its involvement in cell proliferation and apoptosis. Recombinant human PLAGL2 protein can be used to study the effects of PLAGL2 inhibition in cancer cells and to develop novel anti-cancer therapies targeting this protein.

Furthermore, PLAGL2 has been identified as a potential diagnostic and prognostic marker for various cancers, including breast, lung, and colon cancer. Recombinant human PLAGL2 protein can be used in diagnostic tests to detect the expression levels of PLAGL2 in patient samples, which may help in early cancer detection and personalized treatment strategies.

Conclusion

In summary, recombinant human PLAGL2 protein is a crucial transcription factor involved in various cellular processes, including cell proliferation, differentiation, and apoptosis. Its structure, activity, and applications make it a valuable tool for both basic research and clinical applications. Further studies on PLAGL2 may provide insights into its potential as a therapeutic target and diagnostic marker for various diseases, particularly cancer.