Recombinant Human PRKACA Protein, N-His

Description of Recombinant Human PRKACA Protein, N-His

Introduction

Recombinant proteins have become essential tools in various fields of research, diagnostics, and therapeutics. One such protein is the Recombinant Human PRKACA Protein, which has gained significant attention due to its crucial role in cell signaling and its potential applications in various diseases. In this article, we will discuss the structure, activity, and applications of this protein in detail.

Structure of Recombinant Human PRKACA Protein

The Recombinant Human PRKACA Protein, also known as protein kinase A catalytic subunit alpha, is a 39 kDa protein encoded by the PRKACA gene located on chromosome 19. It is a member of the protein kinase A (PKA) family and is composed of 350 amino acids. The protein contains two subunits, the regulatory subunit (PRKAR1A) and the catalytic subunit (PRKACA), which are involved in regulating the activity of PKA.

The catalytic subunit of PRKACA is composed of two domains, the N-terminal domain and the C-terminal domain, connected by a flexible hinge region. The N-terminal domain contains the ATP-binding site, while the C-terminal domain contains the catalytic site responsible for protein phosphorylation. The active form of PRKACA is a homodimer, where the two subunits interact with each other through their N-terminal domains.

Activity of Recombinant Human PRKACA Protein

PRKACA is a serine/threonine kinase that plays a crucial role in various cellular processes, including cell proliferation, differentiation, and apoptosis. It is activated by cyclic adenosine monophosphate (cAMP) binding to the regulatory subunit, which leads to the dissociation of the catalytic subunit from the complex and its activation. Once activated, PRKACA phosphorylates target proteins, including transcription factors, ion channels, and other enzymes, thereby regulating their activity.

The activity of PRKACA is tightly regulated by various mechanisms, including phosphorylation, protein-protein interactions, and subcellular localization. Dysregulation of PRKACA activity has been linked to various diseases, including cancer, cardiovascular disorders, and neurological disorders.

Applications of Recombinant Human PRKACA Protein

The recombinant form of PRKACA has been extensively used in various research areas, including signal transduction, cancer biology, and drug discovery. It has also shown potential in diagnostic and therapeutic applications.

One of the major applications of recombinant PRKACA is in studying cAMP-dependent signaling pathways. Its ability to phosphorylate target proteins has been crucial in understanding the role of PKA in various cellular processes. Additionally, recombinant PRKACA has been used in drug discovery studies to screen for potential inhibitors or activators of PKA activity.

Furthermore, the dysregulation of PRKACA has been implicated in various diseases, making it a potential therapeutic target. Recombinant PRKACA has been used in preclinical studies to evaluate its therapeutic potential in cancer, cardiovascular disorders, and neurological disorders. It has also been used in developing diagnostic assays for these diseases.

Conclusion

The Recombinant Human PRKACA Protein is a crucial component of the cAMP-dependent signaling pathway and plays a significant role in various cellular processes. Its recombinant form has been extensively used in research and has shown potential in diagnostic and therapeutic applications. With further research and development, recombinant PRKACA may hold promise in the treatment of various diseases.