Recombinant Human PRKCG Protein, N-His

Description of Recombinant Human PRKCG Protein, N-His

Introduction

Recombinant Human PRKCG Protein, also known as Protein Kinase C Gamma (PRKCG), is a synthetic form of the human PRKCG protein that is produced through genetic engineering techniques. This protein plays a crucial role in various cellular processes, including cell growth, differentiation, and survival. In this article, we will discuss the structure, activity, and applications of Recombinant Human PRKCG Protein.

Structure of Recombinant Human PRKCG Protein

Recombinant Human PRKCG Protein is a 78-kDa protein composed of 697 amino acids. It belongs to the protein kinase C (PKC) family, which is a group of enzymes that regulate various cellular functions by adding phosphate groups to target proteins. The PRKCG protein has a regulatory domain, a catalytic domain, and a C-terminal domain. The regulatory domain contains two tandem C1 domains, which are responsible for binding to diacylglycerol (DAG) and phorbol esters, two important activators of PRKCG. The catalytic domain contains a highly conserved ATP-binding site, which is essential for the enzymatic activity of PRKCG.

Activity of Recombinant Human PRKCG Protein

Recombinant Human PRKCG Protein is a serine/threonine kinase that plays a crucial role in various cellular processes, including cell growth, differentiation, and survival. It is activated by binding to DAG and phorbol esters, which leads to its translocation from the cytoplasm to the cell membrane. Once activated, PRKCG phosphorylates target proteins, thereby regulating their activity and function. Some of the known substrates of PRKCG include transcription factors, ion channels, and cytoskeletal proteins. Through its activity, PRKCG is involved in the regulation of cell proliferation, apoptosis, and cell migration.

Applications of Recombinant Human PRKCG Protein

Recombinant Human PRKCG Protein has a wide range of applications in both research and therapeutic settings. Here are some of the major applications of this protein:

1. Research Tool

Recombinant Human PRKCG Protein is widely used as a research tool to study the role of PRKCG in various cellular processes. Its ability to be activated by DAG and phorbol esters makes it a useful tool to study the effects of these activators on cellular signaling pathways. In addition, recombinant PRKCG can be used to identify and characterize its substrates, which can provide valuable insights into the functions of this protein.

2. Drug Target

Dysregulation of PRKCG has been linked to several diseases, including cancer, neurological disorders, and cardiovascular diseases. Therefore, recombinant Human PRKCG Protein is being explored as a potential drug target for these diseases. Inhibitors of PRKCG have been developed and tested in preclinical studies, showing promising results in reducing tumor growth and improving neurological function.

3. Diagnostic Marker

The expression of PRKCG has been found to be altered in various diseases, making it a potential diagnostic marker. For example, increased expression of PRKCG has been observed in certain types of cancer, while decreased expression has been linked to Alzheimer’s disease. Therefore, recombinant Human PRKCG Protein can be used as a diagnostic marker to detect these diseases at an early stage.

4. Vaccine Development

Recombinant Human PRKCG Protein has been identified as a potential antigen for vaccine development. It has been shown to induce a strong immune response in animal studies, making it a promising candidate for the development of vaccines against diseases such as cancer and infectious diseases.

5. Cell Signaling Studies

The activity of PRKCG is tightly regulated by various signaling pathways, making it an important player in cell signaling. Recombinant Human PRKCG Protein can be used to study the intricate signaling networks that regulate PR