Recombinant Human SEC22B Protein, N-His

Description of Recombinant Human SEC22B Protein, N-His

Introduction

Recombinant Human SEC22B Protein, also known as SEC22 vesicle trafficking protein-like 2, is a protein that plays a crucial role in intracellular vesicle trafficking and fusion. It is a member of the SEC22 family of proteins, which are involved in the transport of proteins and lipids between different compartments of the cell. Recombinant SEC22B Protein is produced through genetic engineering techniques, making it a valuable tool for various research and therapeutic applications.

Structure of Recombinant Human SEC22B Protein

The SEC22B gene is located on chromosome 1 in humans and encodes for a protein of 232 amino acids. The recombinant version of this protein is produced by inserting the gene into a suitable expression vector and expressing it in a host cell, typically E. coli or yeast. The resulting protein is a soluble protein with a molecular weight of approximately 26 kDa.

SEC22B protein is composed of four domains: the N-terminal domain, the SNARE motif, the transmembrane domain, and the C-terminal domain. The N-terminal domain is responsible for protein-protein interactions and is essential for the fusion of vesicles. The SNARE motif, which is a conserved sequence in all SEC22 family members, is involved in the binding and fusion of vesicles. The transmembrane domain anchors the protein to the membrane, while the C-terminal domain is involved in the regulation of protein function.

Activity of Recombinant Human SEC22B Protein

Recombinant Human SEC22B Protein is involved in the transport of proteins and lipids between the endoplasmic reticulum (ER) and the Golgi apparatus. It is also involved in the fusion of transport vesicles with their target membranes, a process essential for the delivery of cargo to its destination. SEC22B protein is known to interact with other proteins involved in vesicle trafficking, such as SEC22A and SNAP23, to form a complex that mediates vesicle fusion.

Studies have shown that SEC22B protein is essential for the proper functioning of the immune system. It is involved in the transport of major histocompatibility complex (MHC) class II molecules from the ER to the endosomal compartment, where they are loaded with antigens for presentation to T cells. This process is crucial for the activation of T cells, which play a central role in the adaptive immune response.

Application of Recombinant Human SEC22B Protein

Recombinant Human SEC22B Protein has various applications in both research and therapeutic settings. Its role in vesicle trafficking makes it a valuable tool for studying the mechanisms of intracellular transport. It can be used to investigate the role of SEC22B in various cellular processes, such as protein secretion, organelle biogenesis, and cell signaling.

In the field of immunology, recombinant SEC22B protein can be used to study the role of MHC class II molecules in antigen presentation and T cell activation. It can also be used to investigate the mechanisms of immune evasion by pathogens that target the vesicle trafficking pathway.

In a therapeutic setting, SEC22B protein can be used as an antigen for the development of vaccines against infectious diseases. By presenting specific antigens to the immune system, recombinant SEC22B protein can elicit a strong immune response and provide protection against the pathogen. Additionally, SEC22B protein can be used as a target for the development of drugs that can modulate its activity, potentially leading to the treatment of immune-related disorders.

Conclusion

Recombinant Human SEC22B Protein is a crucial player in the intracellular vesicle trafficking pathway, involved in the transport and fusion of vesicles. Its structure and activity make it a valuable tool for studying various cellular processes and its role in the immune system. Its potential applications in research and therapy make it a promising protein for future studies and developments.