Recombinant Human SMYD2 Protein, N-His

Description of Recombinant Human SMYD2 Protein, N-His

Introduction to Recombinant Human SMYD2 Protein

Recombinant Human SMYD2 Protein is a highly purified and bioactive form of the human SMYD2 protein that is produced using recombinant DNA technology. This protein plays a crucial role in various cellular processes such as gene expression, DNA repair, and cell proliferation. In this article, we will delve into the structure, activity, and applications of this protein.

Structure of Recombinant Human SMYD2 Protein

The SMYD2 protein is a member of the SET and MYND domain-containing (SMYD) family of proteins. It is composed of 433 amino acids and has a molecular weight of 49 kDa. The protein contains a SET domain, which is responsible for its histone methyltransferase activity, and a MYND domain, which is involved in protein-protein interactions. The SET domain of SMYD2 is highly conserved and plays a crucial role in its enzymatic activity.

Activity of Recombinant Human SMYD2 Protein

Recombinant Human SMYD2 Protein is a histone methyltransferase, meaning it has the ability to add a methyl group to specific histone proteins. This modification of histones, known as methylation, plays a crucial role in regulating gene expression. SMYD2 specifically targets histone H3 at lysine 4 (H3K4), a modification associated with active gene transcription. By adding a methyl group to H3K4, SMYD2 promotes the recruitment of other transcriptional activators, leading to increased gene expression.

Apart from its histone methyltransferase activity, SMYD2 also has non-histone substrates, including p53, a tumor suppressor protein. SMYD2-mediated methylation of p53 has been shown to regulate its stability and function, thus playing a role in cell proliferation and apoptosis. Additionally, SMYD2 has been reported to methylate other non-histone proteins, such as heat shock protein 90 (HSP90) and retinoblastoma protein (Rb), which are involved in various cellular processes.

Applications of Recombinant Human SMYD2 Protein

The unique activity of Recombinant Human SMYD2 Protein makes it a valuable tool in various research areas. Its ability to specifically target histone H3K4 makes it a useful tool for studying gene expression and epigenetics. The protein can be used to investigate the role of SMYD2 in various cellular processes, such as cell proliferation, DNA repair, and apoptosis.

Moreover, SMYD2 has emerged as a potential therapeutic target in cancer. Its overexpression has been reported in various cancers, including breast, lung, and colon cancer. Inhibiting SMYD2 activity has been shown to decrease cell proliferation and induce apoptosis in cancer cells. Therefore, Recombinant Human SMYD2 Protein can be used to study the mechanism of action of SMYD2 inhibitors and their potential as anti-cancer agents.

In addition to its research applications, Recombinant Human SMYD2 Protein is also used in diagnostic assays. SMYD2 has been identified as a potential biomarker for various diseases, including cancer and cardiovascular diseases. Its levels in blood or tissue samples can be measured using Recombinant Human SMYD2 Protein as a standard, aiding in disease diagnosis and prognosis.

Conclusion

In summary, Recombinant Human SMYD2 Protein is a highly purified and bioactive form of the human SMYD2 protein. Its structure, activity, and applications make it a valuable tool in various research areas, including epigenetics, cancer, and diagnostics. Further studies on this protein and its role in cellular processes could lead to a better understanding of its function and potential therapeutic applications.