Recombinant Human SUZ12 Protein, N-His

Description of Recombinant Human SUZ12 Protein, N-His

Introduction

Recombinant Human SUZ12 Protein is a protein that plays a crucial role in gene regulation and development. It is a key component of the Polycomb Repressive Complex 2 (PRC2), which is responsible for epigenetic silencing of target genes. This protein is produced through recombinant DNA technology, making it a valuable tool for research and therapeutic applications.

Structure of Recombinant Human SUZ12 Protein

The SUZ12 protein is composed of 739 amino acids and has a molecular weight of approximately 83 kDa. It contains a highly conserved zinc finger domain at its N-terminus, which is responsible for binding to histone proteins. The C-terminal region of the protein contains a SET domain, which is involved in histone methyltransferase activity.

The recombinant version of SUZ12 is produced by inserting the gene encoding for the protein into a suitable expression vector, such as a bacterial or mammalian cell system. This allows for the production of large quantities of the protein in a controlled environment.

Activity of Recombinant Human SUZ12 Protein

The main function of SUZ12 is to act as a core component of the PRC2 complex, which is responsible for adding methyl groups to specific histone proteins. This process, known as histone methylation, leads to the silencing of target genes by altering the chromatin structure. SUZ12 is specifically responsible for recognizing and binding to specific DNA sequences, allowing for targeted gene regulation.

In addition to its role in gene regulation, SUZ12 has also been found to play a role in cell differentiation and development. It is essential for the proper development of various tissues and organs, including the central nervous system and the immune system.

Applications of Recombinant Human SUZ12 Protein

The availability of recombinant SUZ12 protein has opened up new avenues for research in the fields of epigenetics and gene regulation. Its ability to specifically target and silence genes has made it a valuable tool for studying the role of specific genes in various biological processes.

Furthermore, the dysregulation of SUZ12 has been linked to various diseases, including cancer. Therefore, recombinant SUZ12 protein has potential applications in the development of targeted therapies for these diseases. It can also be used in drug discovery and screening assays to identify compounds that can modulate the activity of SUZ12.

Conclusion

Recombinant Human SUZ12 Protein is a crucial component of the PRC2 complex, involved in gene regulation and development. Its structure and activity make it a valuable tool for research in the fields of epigenetics and gene regulation. Furthermore, its potential applications in disease treatment and drug discovery make it a promising target for future studies. With the advancement of recombinant DNA technology, the production and use of this protein will continue to contribute to our understanding of gene regulation and its role in various biological processes.

Keywords:

Recombinant protein, antigen, SUZ12, Polycomb Repressive Complex 2, epigenetics, gene regulation, histone methylation, drug discovery, cancer, development.