Recombinant Human TREX1 Protein, N-His-SUMO

Description of Recombinant Human TREX1 Protein, N-His-SUMO

Introduction

Recombinant Human TREX1 Protein is a highly purified protein that is produced through recombinant DNA technology. It is a key enzyme involved in DNA metabolism and has been extensively studied for its role in various cellular processes. In this article, we will explore the structure, activity, and applications of this important protein.

Structure of Recombinant Human TREX1 Protein

The recombinant protein is a 33 kDa protein consisting of 293 amino acids. It belongs to the DNase I-like superfamily and contains a conserved DNase domain at its C-terminus. The protein also has a nuclear localization signal (NLS) at its N-terminus, which is responsible for its transport into the nucleus.

The protein exists as a homodimer in its active form, with each monomer containing a catalytic site responsible for its DNase activity. The homodimeric structure is essential for the enzyme to function properly and is stabilized by several hydrogen bonds and hydrophobic interactions.

Activity of Recombinant Human TREX1 Protein

As mentioned earlier, the recombinant protein is a DNase enzyme that plays a crucial role in DNA metabolism. It is involved in the degradation of single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) in the cytoplasm and nucleus, respectively. The enzyme is highly specific for DNA and does not exhibit any activity towards RNA or other nucleic acids.

Studies have shown that the enzyme is active in a wide range of pH (5.5-8.5) and temperature (25-37°C), making it suitable for various experimental conditions. It also requires divalent cations, such as magnesium or manganese, for its catalytic activity.

Applications of Recombinant Human TREX1 Protein

The recombinant protein has been extensively used in various research applications due to its important role in DNA metabolism. Some of its notable applications are listed below:

1. Study of autoimmune diseases

TREX1 has been implicated in several autoimmune diseases, including Aicardi-Goutières syndrome and systemic lupus erythematosus. The recombinant protein has been used to study the role of TREX1 mutations in these diseases and to understand the underlying mechanisms.

2. Development of diagnostic assays

The enzyme’s specific activity towards DNA has made it a valuable tool for developing diagnostic assays for various diseases. For example, it has been used to detect the presence of viral DNA in patient samples, such as human papillomavirus (HPV) in cervical cancer.

3. DNA damage and repair studies

TREX1 is involved in the degradation of damaged DNA, making it an essential enzyme in DNA repair pathways. The recombinant protein has been used to study the role of TREX1 in DNA damage and repair and to identify potential therapeutic targets for diseases associated with DNA damage.

4. Production of monoclonal antibodies

The recombinant protein has been used as an antigen to produce monoclonal antibodies that specifically recognize TREX1. These antibodies have been used to study the expression and localization of the protein in different cell types and tissues.

5. Drug development

Given the crucial role of TREX1 in various diseases, the recombinant protein has been used in drug discovery and development. It has been used to screen potential inhibitors of the enzyme for the treatment of autoimmune diseases and other disorders associated with TREX1 dysfunction.

Conclusion

In summary, Recombinant Human TREX1 Protein is a key enzyme involved in DNA metabolism with a well-defined structure and activity. Its applications in various research areas make it a valuable tool for studying diseases and developing potential treatments. With ongoing research,