Recombinant Human UCK2 Protein, N-His

Description of Recombinant Human UCK2 Protein, N-His

Introduction to Recombinant Human UCK2 Protein

Recombinant Human UCK2 Protein is a highly specialized protein that plays a crucial role in cellular metabolism. It is a recombinant form of the human UCK2 protein, which is encoded by the UCK2 gene. This protein is involved in the synthesis of nucleoside triphosphates (NTPs), which are essential for various cellular processes such as DNA replication, RNA transcription, and energy metabolism. In this article, we will discuss the structure, activity, and application of Recombinant Human UCK2 Protein.

Structure of Recombinant Human UCK2 Protein

The human UCK2 gene is located on chromosome 1 and consists of 10 exons. The recombinant form of UCK2 protein is produced by cloning the UCK2 gene into a suitable expression vector and expressing it in a host cell. The resulting protein is a 33 kDa enzyme that contains 305 amino acids. It has a conserved nucleoside kinase domain and a unique N-terminal extension, which is essential for its activity.

The crystal structure of Recombinant Human UCK2 Protein has been determined, revealing its active site and substrate binding pocket. The protein has a dimeric structure, with each monomer containing a nucleoside binding site and a magnesium ion, which is essential for its catalytic activity. The active site of the protein is highly conserved among different species, indicating its crucial role in cellular metabolism.

Activity of Recombinant Human UCK2 Protein

The main function of Recombinant Human UCK2 Protein is to catalyze the conversion of nucleoside monophosphates (NMPs) into nucleoside diphosphates (NDPs) using ATP as a phosphate donor. This reaction is essential for the synthesis of NTPs, which are the building blocks of DNA and RNA. The enzyme has a broad substrate specificity and can phosphorylate a wide range of NMPs, including uridine, cytidine, and thymidine.

Recombinant Human UCK2 Protein is also involved in the regulation of cellular energy metabolism. It has been shown to interact with several key enzymes involved in glycolysis and the tricarboxylic acid (TCA) cycle, suggesting its role in the production of ATP, the energy currency of the cell. Additionally, the enzyme has been found to play a crucial role in the activation of certain antiviral and anticancer drugs, making it a potential target for drug development.

Application of Recombinant Human UCK2 Protein

The unique structure and activity of Recombinant Human UCK2 Protein make it a valuable tool in various research areas. One of its main applications is in the study of nucleotide metabolism and its role in various diseases. Mutations in the UCK2 gene have been linked to several disorders, including mitochondrial diseases and cancer. Recombinant Human UCK2 Protein can be used to study the effects of these mutations on the activity and function of the enzyme.

The enzyme is also used in drug discovery and development, particularly in the field of antiviral and anticancer drugs. Its ability to activate certain drugs, such as 5-fluorouracil, makes it a potential therapeutic target for the treatment of cancer. Additionally, Recombinant Human UCK2 Protein can be used to screen for potential inhibitors of the enzyme, which could be developed into new drugs for the treatment of various diseases.

Conclusion

Recombinant Human UCK2 Protein is a highly specialized enzyme that plays a crucial role in cellular metabolism. Its unique structure and activity make it a valuable tool in various research areas, including nucleotide metabolism and drug discovery. Further studies on this protein could lead to a better understanding of its function and potential therapeutic applications.