XtenCHO ™ Race Starter kit

Description of XtenCHO ™ Race Starter kit

XtenCHO™ Race Mammalian Expression System

The XtenCHO™ Race mammalian expression system has been meticulously developed to provide a swift and economical solution for recombinant protein expression. It is particularly suited for a diverse array of applications, ranging from small- to medium-scale production and the expedited screening of drug candidates and protein variants.

Leveraging over 28 years of expertise, XtenCHO™ Race integrates the high expression capabilities and resilience of a novel Chinese Hamster Ovary (CHO) cell line with the superior efficiency of our proprietary transfection reagent, XtenFect™. This combination yields numerous advantages, including:
• Yield increases of up to 10 times compared to other commercial systems*
• Enhanced plasmid retention (over 14 days of transient gene expression – TGE) achieved through highly effective transfection that maintains cell viability, improves plasmid stability, and elevates protein expression without requiring additional feeds
• Short protocol in 8 days only
• Streamlined protocols with fewer steps, designed to simplify and expedite your workflow, supported by robust and responsive technical assistance
• Elimination of case-by-case optimization due to the high expression levels of the pXen1 vector
• Optimal protein folding and human-like post-translational modifications
• Cost-effective biopharmaceutical-grade production
• Improved safety owing to the enhanced viral resistance of CHO cells in comparison to Human Embryonic Kidney (HEK) cells
• Flexible and scalable production capabilities (ranging from 1 mL to 10 L) tailored to meet your specific requirements
• Worldwide shipping within 24 hours or less

In comparison to other commercial systems, XtenCHO™ has demonstrated superior performance, achieving a yield increase of 1.5 to 9.7-fold*:
• Record productivity for IgG-like antibodies (1.8 g/L for Garetosmab)
• Bispecific antibodies exceeding 100 mg/L
• Humanized antibody variants surpassing 200 mg/L
• Non-antibody proteins yielding over 100 mg/L for ACE2 with excellent enzymatic activity
• Pentameric IgM antibodies
• And more

The XtenCHO™ Race kit features our exclusive chemically defined, animal-free expression media designed to ensure optimal performance. It also includes our effective expression booster, XtenCHO™ Enhancer, along with control plasmids to provide maximum control and flexibility.

Why choose XtenCHO™ for your biopharmaceutical research projects?

For many years, biomolecules entering phase I clinical trials have experienced a low success rate of under 10%. Nevertheless, nearly three-quarters of biopharmaceuticals that have received approval are produced using CHO-based systems. An analysis of the data indicates that CHO-based systems may effectively address early developmental challenges that commonly impede success.
XtenCHO™ Race enables the production of high-quality recombinant proteins for comprehensive early bioanalysis, allowing for the early identification of potential developability issues and enhancing your likelihood of success.
With ultra-high yields, rapid production, and protein folding and P&trade comparable to clinical-grade biopharmaceuticals, XtenCHO™ is exceptionally well-suited for the swift evaluation of drug candidates or protein variants generated through random or targeted mutagenesis.

Why utilize XtenCHO™ Race for your research applications?

Although bioassays serve as essential research and monitoring tools across various industries—including medicine, food production, and environmental analysis—they often depend on antibodies produced natively in hybridomas, resulting in lower purity and limited accuracy and precision. However, the landscape is evolving. Through its CHO-based recombinant expression, XtenCHO™ Race enables you to achieve higher yields, enhance batch-to-batch consistency, and improve purity in a cost-effective manner. With intelligent protocols, XtenCHO™ Race will integrate smoothly into your in-house workflows and adapt to your protein production requirements.

XtenCHO™ Race outperforms up to 6X (Cixutumumab and Peresolimab)

Crude production yields in mg/L obtained in 24-well plates cultures without feeding (Octet Analysis) Yields in flask (better aeration) will be 1.5 to 2.5 Higher for Human/humanized IgG1/IgG4 (depending on the antibody considered)