XtenCHO ™ Race Transfection kit

Description of XtenCHO ™ Race Transfection kit

Transfection: A Key Step in Recombinant Protein Production

Transfection is a critical process in recombinant protein production using eukaryotic cells. This technique introduces nucleic acid sequences into cells, enabling them to harness their own cellular machinery for protein expression. However, achieving efficient transfection can be challenging due to high costs and potential impacts on cell viability. To address these challenges, various technologies have been developed to enhance transfection success.

ProteoGenix has designed XtenCHO™ Race, a proprietary CHO-based cell line optimized for recombinant protein production. To further support researchers, the XtenCHO™ Race Transfection Kit has been specifically engineered to maximize the transient transfection efficiency of XtenCHO™ Race cells, ensuring exceptionally high protein yields with optimized workflow efficiency.

XtenCHO™ Race Transfection Kit: Optimized for Maximum Yields

The XtenCHO™ Race Transfection Kit includes two key components:

-XtenFect Reagent: Designed specifically for transfecting XtenCHO™ Race cell cultures grown in XtenCHO™ Expression Medium, ensuring efficient nucleic acid uptake by CHO cells. This step is essential for maximizing recombinant protein production within the XtenCHO™ Expression System.
-XtenCHO™ Race Enhancer: Maintains high cell viability even in densely transfected cultures, extending protein production time and increasing yield per liter of culture.

The XtenFect Reagent is supplied as a 10x concentrated stock solution, which must be diluted using the included XtenFect Working Solution. Once prepared, the XtenFect Working Solution can be directly added to XtenCHO™ Race cultures—eliminating the need for pre-mixing with DNA, a common step in other transfection methods. This streamlined protocol significantly simplifies the transfection process, saving researchers time while boosting efficiency.

Designed for 1 liter of culture, the XtenCHO™ Race Transfection Kit is completely animal-component free, formulated exclusively with chemically defined ingredients for optimal reproducibility and regulatory compliance.

The XtenCHO™ Race Transfection Kit is also an integral part of the XtenCHO™ Race Starter Kit, offering researchers a comprehensive solution for high-yield recombinant protein production.

XtenCHO™ Race outperforms up to 6X (Cixutumumab and Peresolimab)

Crude production yields in mg/L obtained in 24-well plates cultures without feeding (Octet Analysis) Yields in flask (better aeration) will be 1.5 to 2.5 Higher for Human/humanized IgG1/IgG4 (depending on the antibody considered)